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000482764 1001_ $$aHolmes, Susannah$$b0
000482764 245__ $$aMegahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers
000482764 260__ $$a[London]$$bNature Publishing Group UK$$c2022
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000482764 520__ $$aThe European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive X-ray pulses to collect diffraction patterns from individual crystals. Here, we exploit the MHz pulse structure of the European XFEL to obtain two complete datasets from the same lysozyme crystal, first hit and the second hit, before it exits the beam. The two datasets, separated by <1 µs, yield up to 2.1 Å resolution structures. Comparisons between the two structures reveal no indications of radiation damage or significant changes within the active site, consistent with the calculated dose estimates. This demonstrates MHz SFX can be used as a tool for tracking sub-microsecond structural changes in individual single crystals, a technique we refer to as multi-hit SFX.
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000482764 536__ $$0G:(GEPRIS)390715994$$aDFG project 390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994)$$c390715994$$x2
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000482764 7001_ $$aKirkwood, Henry J.$$b1
000482764 7001_ $$aBean, Richard$$b2
000482764 7001_ $$aGiewekemeyer, Klaus$$b3
000482764 7001_ $$aMartin, Andrew V.$$b4
000482764 7001_ $$aHadian-Jazi, Marjan$$b5
000482764 7001_ $$aWiedorn, Max O.$$b6
000482764 7001_ $$aOberthür, Dominik$$b7
000482764 7001_ $$aMarman, Hugh$$b8
000482764 7001_ $$aAdriano, Luigi$$b9
000482764 7001_ $$aAl-Qudami, Nasser$$b10
000482764 7001_ $$0P:(DE-H253)PIP1006443$$aBajt, Saša$$b11
000482764 7001_ $$aBarák, Imrich$$b12
000482764 7001_ $$0P:(DE-H253)PIP1014119$$aBari, Sadia$$b13
000482764 7001_ $$aBielecki, Johan$$b14
000482764 7001_ $$aBrockhauser, Sandor$$b15
000482764 7001_ $$aColeman, Mathew A.$$b16
000482764 7001_ $$aCruz-Mazo, Francisco$$b17
000482764 7001_ $$aDanilevski, Cyril$$b18
000482764 7001_ $$aDörner, Katerina$$b19
000482764 7001_ $$aGañán-Calvo, Alfonso M.$$b20
000482764 7001_ $$aGraceffa, Rita$$b21
000482764 7001_ $$00000-0001-5494-7193$$aFanghor, Hans$$b22
000482764 7001_ $$aHeymann, Michael$$b23
000482764 7001_ $$aFrank, Matthias$$b24
000482764 7001_ $$aKaukher, Alexander$$b25
000482764 7001_ $$aKim, Yoonhee$$b26
000482764 7001_ $$aKobe, Bostjan$$b27
000482764 7001_ $$aKnoška, Juraj$$b28
000482764 7001_ $$aLaurus, Torsten$$b29
000482764 7001_ $$aLetrun, Romain$$b30
000482764 7001_ $$aMaia, Luis$$b31
000482764 7001_ $$aMesserschmidt, Marc$$b32
000482764 7001_ $$aMetz, Markus$$b33
000482764 7001_ $$aMichelat, Thomas$$b34
000482764 7001_ $$aMills, Grant$$b35
000482764 7001_ $$0P:(DE-H253)PIP1007192$$aMolodtsov, Serguei$$b36
000482764 7001_ $$aMonteiro, Diana C. F.$$b37
000482764 7001_ $$aMorgan, Andrew J.$$b38
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000482764 7001_ $$0P:(DE-H253)PIP1020365$$aPeña Murillo, Gisel E.$$b40
000482764 7001_ $$0P:(DE-H253)PIP1030261$$aPrevitali, Gianpietro$$b41
000482764 7001_ $$aRound, Adam$$b42
000482764 7001_ $$aSato, Tokushi$$b43
000482764 7001_ $$0P:(DE-H253)PIP1018164$$aSchubert, Robin$$b44
000482764 7001_ $$0P:(DE-H253)PIP1006337$$aSchulz, Joachim$$b45
000482764 7001_ $$aShelby, Megan$$b46
000482764 7001_ $$0P:(DE-H253)PIP1020921$$aSeuring, Carolin$$b47
000482764 7001_ $$aSellberg, Jonas A.$$b48
000482764 7001_ $$0P:(DE-H253)PIP1004233$$aSikorski, Marcin$$b49
000482764 7001_ $$aSilenzi, Alessandro$$b50
000482764 7001_ $$aStern, Stephan$$b51
000482764 7001_ $$aSztuk-Dambietz, Jola$$b52
000482764 7001_ $$aSzuba, Janusz$$b53
000482764 7001_ $$0P:(DE-H253)PIP1011270$$aTrebbin, Martin$$b54
000482764 7001_ $$aVagovic, Patrick$$b55
000482764 7001_ $$aVe, Thomas$$b56
000482764 7001_ $$aWeinhausen, Britta$$b57
000482764 7001_ $$aWrona, Krzysztof$$b58
000482764 7001_ $$aXavier, Paul Lourdu$$b59
000482764 7001_ $$aXu, Chen$$b60
000482764 7001_ $$aYefanov, Oleksandr$$b61
000482764 7001_ $$aNugent, Keith A.$$b62
000482764 7001_ $$0P:(DE-H253)PIP1006324$$aChapman, Henry N.$$b63
000482764 7001_ $$aMancuso, Adrian P.$$b64
000482764 7001_ $$0P:(DE-H253)PIP1008245$$aBarty, Anton$$b65
000482764 7001_ $$0P:(DE-HGF)0$$aAbbey, Brian$$b66$$eCorresponding author
000482764 7001_ $$0P:(DE-HGF)0$$aDarmanin, Connie$$b67$$eCorresponding author
000482764 773__ $$0PERI:(DE-600)2553671-0$$a10.1038/s41467-022-32434-6$$gVol. 13, no. 1, p. 4708$$n1$$p4708$$tNature Communications$$v13$$x2041-1723$$y2022
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