Journal Article

PUBDB-2016-06046

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Use of small-angle X-ray scattering to resolve intracellular structure changes of Escherichia coli cells induced by antibiotic treatment

von Gundlach, A. R. ; Garamus, V. M. ; Willey, T. M. ; Ilavsky, J. ; Hilpert, K. ; Rosenhahn, A. (Corresponding author)Extern*

2016
Munksgaard Copenhagen

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Please use a persistent id in citations: doi:10.1107/S1600576716018562  doi:10.3204/PUBDB-2016-06046

Abstract: The application of small-angle X-ray scattering (SAXS) to whole Escherichia coli cells is challenging owing to the variety of internal constituents. To resolve their contributions, the outer shape was captured by ultra-small-angle X-ray scattering and combined with the internal structure resolved by SAXS. Building on these data, a model for the major structural components of E. coli was developed. It was possible to deduce information on the occupied volume, occurrence and average size of the most important intracellular constituents:ribosomes, DNA and proteins. E. coli was studied after treatment with three different antibiotic agents (chloramphenicol, tetracycline and rifampicin) and the impact on the intracellular constituents was monitored.

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Contributing Institute(s):

1. EMBL-User (EMBL-User)
2. DOOR-User (DOOR)

Research Program(s):

1. 6G3 - PETRA III (POF3-622) (POF3-622)
2. VH-VI-403 - In-Situ Nano-Imaging of Biological and Chemical Processes (2015_IFV-VH-VI-403) (2015_IFV-VH-VI-403)

Experiment(s):

1. PETRA Beamline P12 (PETRA III)

Appears in the scientific report 2016

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Database coverage:
; ; ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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