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@ARTICLE{vonGundlach:315721,
author = {von Gundlach, A. R. and Garamus, V. M. and Willey, T. M.
and Ilavsky, J. and Hilpert, K. and Rosenhahn, A.},
title = {{U}se of small-angle {X}-ray scattering to resolve
intracellular structure changes of {E}scherichia coli cells
induced by antibiotic treatment},
journal = {Journal of applied crystallography},
volume = {49},
number = {6},
issn = {1600-5767},
address = {Copenhagen},
publisher = {Munksgaard},
reportid = {PUBDB-2016-06046},
pages = {2210 - 2216},
year = {2016},
abstract = {The application of small-angle X-ray scattering (SAXS) to
whole Escherichia coli cells is challenging owing to the
variety of internal constituents. To resolve their
contributions, the outer shape was captured by
ultra-small-angle X-ray scattering and combined with the
internal structure resolved by SAXS. Building on these data,
a model for the major structural components of E. coli was
developed. It was possible to deduce information on the
occupied volume, occurrence and average size of the most
important intracellular constituents:ribosomes, DNA and
proteins. E. coli was studied after treatment with three
different antibiotic agents (chloramphenicol, tetracycline
and rifampicin) and the impact on the intracellular
constituents was monitored.},
cin = {EMBL-User / DOOR},
ddc = {540},
cid = {I:(DE-H253)EMBL-User-20120814 /
I:(DE-H253)HAS-User-20120731},
pnm = {6G3 - PETRA III (POF3-622) / VH-VI-403 - In-Situ
Nano-Imaging of Biological and Chemical Processes
$(2015_IFV-VH-VI-403)$},
pid = {G:(DE-HGF)POF3-6G3 / $G:(DE-HGF)2015_IFV-VH-VI-403$},
experiment = {EXP:(DE-H253)P-P12-20150101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000391195900037},
pubmed = {pmid:27980516},
doi = {10.1107/S1600576716018562},
url = {https://bib-pubdb1.desy.de/record/315721},
}