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000315721 1001_ $$0P:(DE-HGF)0$$avon Gundlach, A. R.$$b0
000315721 245__ $$aUse of small-angle X-ray scattering to resolve intracellular structure changes of Escherichia coli cells induced by antibiotic treatment
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000315721 520__ $$aThe application of small-angle X-ray scattering (SAXS) to whole Escherichia coli cells is challenging owing to the variety of internal constituents. To resolve their contributions, the outer shape was captured by ultra-small-angle X-ray scattering and combined with the internal structure resolved by SAXS. Building on these data, a model for the major structural components of E. coli was developed. It was possible to deduce information on the occupied volume, occurrence and average size of the most important intracellular constituents:ribosomes, DNA and proteins. E. coli was studied after treatment with three different antibiotic agents (chloramphenicol, tetracycline and rifampicin) and the impact on the intracellular constituents was monitored.
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000315721 7001_ $$0P:(DE-HGF)0$$aGaramus, V. M.$$b1
000315721 7001_ $$0P:(DE-HGF)0$$aWilley, T. M.$$b2
000315721 7001_ $$0P:(DE-HGF)0$$aIlavsky, J.$$b3
000315721 7001_ $$0P:(DE-HGF)0$$aHilpert, K.$$b4
000315721 7001_ $$0P:(DE-H253)PIP1010951$$aRosenhahn, A.$$b5$$eCorresponding author
000315721 773__ $$0PERI:(DE-600)2020879-0$$a10.1107/S1600576716018562$$gVol. 49, no. 6, p. 2210 - 2216$$n6$$p2210 - 2216$$tJournal of applied crystallography$$v49$$x1600-5767$$y2016
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