Journal Article

PUBDB-2025-04027

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Nucleotide-bound crystal structures of the SARS-CoV-2 helicase NSP13

Kloskowski, P. ; Neumann, P. ; Berndt, A. ; Ficner, R. (Corresponding author)Extern*EMBL*

2025
Blackwell Oxford [u.a.]

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Please use a persistent id in citations: doi:10.1107/S2053230X25005266  doi:10.3204/PUBDB-2025-04027

Abstract: Nucleotide-bound crystal structures of SARS-CoV-2 NSP13 in ADP- and ATP-bound states were resolved to 1.8 and 1.9 Å, respectively. The ADP-bound model captures a state immediately following ATP hydrolysis, with both ADP and orthophosphate still present in the active site. Further comparative analysis revealed that crystal packing influences NSP13 by stabilizing the nucleotide-binding site, underscoring the importance of accounting for these effects in structure-based drug design targeting NSP13.

Note: Funding for this research was provided by the DeutscheForschungsgemeinschaft (Germany’s Excellence StrategyEXC 2067/1-390729940 to RF and grant No. INST186/1117 toRF).

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Contributing Institute(s):

1. EMBL-User (EMBL-User)

Research Program(s):

1. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
2. DFG project G:(GEPRIS)390729940 - EXC 2067: Multiscale Bioimaging: Von molekularen Maschinen zu Netzwerken erregbarer Zellen (390729940) (390729940)

Experiment(s):

1. PETRA Beamline P13 (PETRA III)

Appears in the scientific report 2025

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; DEAL Wiley ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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