Journal Article

PUBDB-2025-02028

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Nuclear pore permeability and fluid flow are modulated by its dilation state

Hoffmann, P. C. ; Kim, H. ; Obarska-Kosinska, A. ; Kreysing, J. P. ; Andino-Frydman, E. ; Cruz-León, S. ; Margiotta, E. ; Cernikova, L.Extern*CSSB* ; Kosinski, J.CSSB* ; Turoňová, B. ; Hummer, G. (Corresponding author) ; Beck, M. (Corresponding author)

2025
Cell Press [Cambridge, Mass.]

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Please use a persistent id in citations: doi:10.1016/j.molcel.2024.11.038

Abstract: Changing environmental conditions necessitate an immediate cellular adaptation to ensure survival. Dictyostelium discoideum, a bacteriovore slime mold present in the soil of most terrestrial ecosystems, is known for its ability to tolerate drastic changes in osmolarity. How the cells cope with the resulting mechanical stress remains understudied. Here we show that D. discoideum has extraordinarily elaborate and resilient nuclear pores that serve as conduits for massive fluid exchange between cytosol and nucleus. We capitalize on the unique properties of D. discoideum cells to quantify flow across the nuclear envelope that is necessitated by changing nuclear size in response to osmotic stress. Based on mathematical concepts adapted from hydrodynamics, we conceptualize this phenomenon as porous flow across nuclear pores. This type of fluid flow is distinct from the canonically characterized modes of nucleocytoplasmic transport, i.e. passive diffusion and active nuclear transport, because of its dependence on pressure. Our insights are relevant in any biological condition that necessitates rapid nuclear size changes, which includes metastasizing cancer cells squeezing through constrictions, migrating cells and differentiating tissues.

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Contributing Institute(s):

1. CSSB-EMBL-JK (CSSB-EMBL-JK)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Experiment(s):

1. No specific instrument

Appears in the scientific report 2025

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 15 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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Linked articles:

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Preprint Hoffmann, P. C. ; Kim, H. ; Obarska-Kosinska, A. ; Kreysing, J. P. ; Andino-Frydman, E. ; Cruz-Leon, S. ; Cernikova, L. ; Kosinski, J. ; Turoňová, B. ; Hummer, G. (Corresponding author) ; Beck, M. (Corresponding author)
Nuclear pores as conduits for fluid flow during osmotic stress
[10.1101/2024.01.17.575985]     Files BibTeX | EndNote: XML, Text | RIS

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