TY  - JOUR
AU  - Hoffmann, Patrick C.
AU  - Kim, Hyuntae
AU  - Obarska-Kosinska, Agnieszka
AU  - Kreysing, Jan Philipp
AU  - Andino-Frydman, Eli
AU  - Cruz-León, Sergio
AU  - Margiotta, Erica
AU  - Cernikova, Lenka
AU  - Kosinski, Jan
AU  - Turoňová, Beata
AU  - Hummer, Gerhard
AU  - Beck, Martin
TI  - Nuclear pore permeability and fluid flow are modulated by its dilation state
JO  - Molecular cell
VL  - 85
IS  - 3
SN  - 1097-2765
CY  - [Cambridge, Mass.]
PB  - Cell Press
M1  - PUBDB-2025-02028
SP  - 537 - 554.e11
PY  - 2025
AB  - Changing environmental conditions necessitate an immediate cellular adaptation to ensure survival. Dictyostelium discoideum, a bacteriovore slime mold present in the soil of most terrestrial ecosystems, is known for its ability to tolerate drastic changes in osmolarity. How the cells cope with the resulting mechanical stress remains understudied. Here we show that D. discoideum has extraordinarily elaborate and resilient nuclear pores that serve as conduits for massive fluid exchange between cytosol and nucleus. We capitalize on the unique properties of D. discoideum cells to quantify flow across the nuclear envelope that is necessitated by changing nuclear size in response to osmotic stress. Based on mathematical concepts adapted from hydrodynamics, we conceptualize this phenomenon as porous flow across nuclear pores. This type of fluid flow is distinct from the canonically characterized modes of nucleocytoplasmic transport, i.e. passive diffusion and active nuclear transport, because of its dependence on pressure. Our insights are relevant in any biological condition that necessitates rapid nuclear size changes, which includes metastasizing cancer cells squeezing through constrictions, migrating cells and differentiating tissues.
LB  - PUB:(DE-HGF)16
C6  - pmid:39729993
DO  - DOI:10.1016/j.molcel.2024.11.038
UR  - https://bib-pubdb1.desy.de/record/631516
ER  -