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@ARTICLE{Hoffmann:631516,
author = {Hoffmann, Patrick C. and Kim, Hyuntae and Obarska-Kosinska,
Agnieszka and Kreysing, Jan Philipp and Andino-Frydman, Eli
and Cruz-León, Sergio and Margiotta, Erica and Cernikova,
Lenka and Kosinski, Jan and Turoňová, Beata and Hummer,
Gerhard and Beck, Martin},
title = {{N}uclear pore permeability and fluid flow are modulated by
its dilation state},
journal = {Molecular cell},
volume = {85},
number = {3},
issn = {1097-2765},
address = {[Cambridge, Mass.]},
publisher = {Cell Press},
reportid = {PUBDB-2025-02028},
pages = {537 - 554.e11},
year = {2025},
abstract = {Changing environmental conditions necessitate an immediate
cellular adaptation to ensure survival. Dictyostelium
discoideum, a bacteriovore slime mold present in the soil of
most terrestrial ecosystems, is known for its ability to
tolerate drastic changes in osmolarity. How the cells cope
with the resulting mechanical stress remains understudied.
Here we show that D. discoideum has extraordinarily
elaborate and resilient nuclear pores that serve as conduits
for massive fluid exchange between cytosol and nucleus. We
capitalize on the unique properties of D. discoideum cells
to quantify flow across the nuclear envelope that is
necessitated by changing nuclear size in response to osmotic
stress. Based on mathematical concepts adapted from
hydrodynamics, we conceptualize this phenomenon as porous
flow across nuclear pores. This type of fluid flow is
distinct from the canonically characterized modes of
nucleocytoplasmic transport, i.e. passive diffusion and
active nuclear transport, because of its dependence on
pressure. Our insights are relevant in any biological
condition that necessitates rapid nuclear size changes,
which includes metastasizing cancer cells squeezing through
constrictions, migrating cells and differentiating tissues.},
cin = {CSSB-EMBL-JK},
ddc = {610},
cid = {I:(DE-H253)CSSB-EMBL-JK-20210701},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
experiment = {EXP:(DE-MLZ)NOSPEC-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:39729993},
doi = {10.1016/j.molcel.2024.11.038},
url = {https://bib-pubdb1.desy.de/record/631516},
}