Journal Article

PUBDB-2024-08044

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Unveiling the cell biology of hippocampal neurons with dendritic axon origin

Han, Y. ; Hacker, D. ; Donders, B. C. ; Parperis, C. ; Thuenauer, R.Extern*CSSB* ; Leterrier, C. ; Grünewald, K. (Corresponding author) ; Mikhaylova, M. (Corresponding author)

2025
Rockefeller Univ. Press New York, NY

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Please use a persistent id in citations: doi:10.1083/jcb.202403141  doi:10.3204/PUBDB-2024-08044

Abstract: In mammalian axon-carrying–dendrite (AcD) neurons, the axon emanates from a basal dendrite, instead of the soma, to create a privileged route for action potential generation at the axon initial segment (AIS). However, it is unclear how such unusual morphology is established and whether the structure and function of the AIS in AcD neurons are preserved. By using dissociated hippocampal cultures as a model, we show that the development of AcD morphology can occur prior to synaptogenesis and independently of the in vivo environment. A single precursor neurite first gives rise to the axon and then to the AcD. The AIS possesses a similar cytoskeletal architecture as the soma-derived AIS and similarly functions as a trafficking barrier to retain axon-specific molecular composition. However, it does not undergo homeostatic plasticity, contains lesser cisternal organelles, and receives fewer inhibitory inputs. Our findings reveal insights into AcD neuron biology and underscore AIS structural differences based on axon onset.

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Contributing Institute(s):

1. CSSB - Leibniz-Institut für Experimentelle Virologie (LIV) - Kay Grünewald (CSSB-LIV-KG)
2. CSSB-CF-ALFM (CSSB-CF-ALFM)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Experiment(s):

1. No specific instrument
2. (Advanced Light and Fluorescence Microscopy Facility)

Appears in the scientific report 2025

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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