Journal Article PUBDB-2024-00792

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png
Structural basis for subversion of host cell actin cytoskeleton during Salmonella infection

 ;  ;  ;  ;  ;  ;  ;  ;

2023
Assoc. Washington, DC [u.a.]

Science advances 9(49), eadj5777 () [10.1126/sciadv.adj5777]
 GO

This record in other databases:        

Please use a persistent id in citations: doi:  doi:

Abstract: Secreted bacterial type III secretion system (T3SS) proteins are essential for successful infection by many human pathogens. Both T3SS translocator SipC and effector SipA are critical for Salmonella infection by subversion of the host cell cytoskeleton, but the precise molecular interplay between them remains unknown. Here, using cryo–electron microscopy, we show that SipA binds along the F-actin grooves with a unique binding pattern. SipA stabilizes F-actin through charged interface residues and appears to prevent inorganic phosphate release through closure of the “back door” of adenosine 5′-triphosphate pocket. We also show that SipC enhances the binding of SipA to F-actin, thus demonstrating that a sequential presence of T3SS proteins in host cells is associated with a sequence of infection events—starting with actin nucleation, filament growth, and stabilization. Together, our data explain the coordinated interplay of a precisely tuned and highly effective mechanism during Salmonella infection and provide a blueprint for interfering with Salmonella effectors acting on actin.

Classification:

Note: DFG Fa330/12-3. Part of this work was performed at the Multi-User Cryo-EM Facility at the Centre for Structural Systems Biology, Hamburg, supported by the University of Hamburg and DFG grant numbers (INST 152/772-1, 152/774-1, 152/775-1, 152/776-1, and 152/777-1 FUGG).

Contributing Institute(s):
  1. CSSB-UKE-TM (CSSB-UKE-TM)
  2. CSSB-CF-ALFM (CSSB-CF-ALFM)
Research Program(s):
  1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
Experiment(s):
  1. No specific instrument

Database coverage:
Medline ; Creative Commons Attribution-NonCommercial CC BY-NC 4.0 ; DOAJ ; OpenAccess ; Article Processing Charges ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; Ebsco Academic Search ; Essential Science Indicators ; Fees ; IF >= 10 ; JCR ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record
Click to display QR Code for this record

The record appears in these collections:
Private Collections > >CSSB > CSSB-CF-ALFM
Private Collections > >CSSB > CSSB-UKE-TM
Document types > Articles > Journal Article
Public records
Publications database
OpenAccess

 Record created 2024-02-15, last modified 2025-07-15


OpenAccess:
Download fulltext PDF Download fulltext PDF (PDFA)
Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)