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000603155 1001_ $$0P:(DE-H253)PIP1091457$$aYuan, Biao$$b0
000603155 245__ $$aStructural basis for subversion of host cell actin cytoskeleton during Salmonella infection
000603155 260__ $$aWashington, DC [u.a.]$$bAssoc.$$c2023
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000603155 500__ $$aDFG Fa330/12-3. Part of this work was performed at the Multi-User Cryo-EM Facility at the Centre for Structural Systems Biology, Hamburg, supported by the University of Hamburg and DFG grant numbers (INST 152/772-1, 152/774-1, 152/775-1, 152/776-1, and 152/777-1 FUGG). 
000603155 520__ $$aSecreted bacterial type III secretion system (T3SS) proteins are essential for successful infection by many human pathogens. Both T3SS translocator SipC and effector SipA are critical for Salmonella infection by subversion of the host cell cytoskeleton, but the precise molecular interplay between them remains unknown. Here, using cryo–electron microscopy, we show that SipA binds along the F-actin grooves with a unique binding pattern. SipA stabilizes F-actin through charged interface residues and appears to prevent inorganic phosphate release through closure of the “back door” of adenosine 5′-triphosphate pocket. We also show that SipC enhances the binding of SipA to F-actin, thus demonstrating that a sequential presence of T3SS proteins in host cells is associated with a sequence of infection events—starting with actin nucleation, filament growth, and stabilization. Together, our data explain the coordinated interplay of a precisely tuned and highly effective mechanism during Salmonella infection and provide a blueprint for interfering with Salmonella effectors acting on actin. 
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000603155 7001_ $$0P:(DE-HGF)0$$aScholz, Jonas$$b1
000603155 7001_ $$0P:(DE-H253)PIP1083333$$aWald, Jiri$$b2
000603155 7001_ $$0P:(DE-H253)PIP1087856$$aThünauer, Roland$$b3
000603155 7001_ $$0P:(DE-H253)PIP1099073$$aHennell James, Rory$$b4
000603155 7001_ $$0P:(DE-HGF)0$$aEllenberg, Irina$$b5
000603155 7001_ $$0P:(DE-HGF)0$$aWindhorst, Sabine$$b6
000603155 7001_ $$0P:(DE-HGF)0$$aFaix, Jan$$b7
000603155 7001_ $$0P:(DE-H253)PIP1021412$$aMarlovits, Thomas$$b8$$eCorresponding author
000603155 773__ $$0PERI:(DE-600)2810933-8$$a10.1126/sciadv.adj5777$$gVol. 9, no. 49, p. eadj5777$$n49$$peadj5777$$tScience advances$$v9$$x2375-2548$$y2023
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