Journal Article

PUBDB-2024-00265

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png A malaria parasite phospholipase facilitates efficient asexual blood stage egress

Ramaprasad, A. ; Burda, P.-C.CSSB* ; Koussis, K. ; Thomas, J. A. ; Pietsch, E.CSSB* ; Calvani, E. ; Howell, S. A. ; MacRae, J. I. ; Snijders, A. P. ; Gilberger, T.-W.CSSB* ; Blackman, M. J. (Corresponding author)

2023
PLoS Lawrence, Kan.

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Please use a persistent id in citations: doi:10.1371/journal.ppat.1011449  doi:10.3204/PUBDB-2024-00265

Abstract: Malaria parasite release (egress) from host red blood cells involves parasite-mediated membrane poration and rupture, thought to involve membrane-lytic effector molecules such as perforin-like proteins and/or phospholipases. With the aim of identifying these effectors, we disrupted the expression of two Plasmodium falciparum perforin-like proteins simultaneously and showed that they have no essential roles during blood stage egress. Proteomic profiling of parasite proteins discharged into the parasitophorous vacuole (PV) just prior to egress detected the presence in the PV of a lecithin:cholesterol acyltransferase (LCAT; PF3D7_0629300). Conditional ablation of LCAT resulted in abnormal egress and a reduced replication rate. Lipidomic profiles of LCAT-null parasites showed drastic changes in several phosphatidylserine and acylphosphatidylglycerol species during egress. We thus show that, in addition to its previously demonstrated role in liver stage merozoite egress, LCAT is required to facilitate efficient egress in asexual blood stage malaria parasites.

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Contributing Institute(s):

1. CSSB-BNITM-TG (CSSB-BNITM-TG)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)
2. MalariaEgress - Role of perforin-like proteins and phospholipases in malaria parasite egress (751865) (751865)
3. DFG project G:(GEPRIS)414222880 - Funktionelle Charakterisierung der Phospholipasen im Malariaerreger Plasmodium falciparum (414222880) (414222880)

Experiment(s):

1. No specific instrument

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