TY  - JOUR
AU  - Ramaprasad, Abhinay
AU  - Burda, Paul-Christian
AU  - Koussis, Konstantinos
AU  - Thomas, James A.
AU  - Pietsch, Emma
AU  - Calvani, Enrica
AU  - Howell, Steven A.
AU  - MacRae, James I.
AU  - Snijders, Ambrosius P.
AU  - Gilberger, Tim-Wolf
AU  - Blackman, Michael J.
TI  - A malaria parasite phospholipase facilitates efficient asexual blood stage egress
JO  - PLoS pathogens
VL  - 19
IS  - 6
SN  - 1553-7366
CY  - Lawrence, Kan.
PB  - PLoS
M1  - PUBDB-2024-00265
SP  - e1011449 
PY  - 2023
AB  - Malaria parasite release (egress) from host red blood cells involves parasite-mediated membrane poration and rupture, thought to involve membrane-lytic effector molecules such as perforin-like proteins and/or phospholipases. With the aim of identifying these effectors, we disrupted the expression of two Plasmodium falciparum perforin-like proteins simultaneously and showed that they have no essential roles during blood stage egress. Proteomic profiling of parasite proteins discharged into the parasitophorous vacuole (PV) just prior to egress detected the presence in the PV of a lecithin:cholesterol acyltransferase (LCAT; PF3D7_0629300). Conditional ablation of LCAT resulted in abnormal egress and a reduced replication rate. Lipidomic profiles of LCAT-null parasites showed drastic changes in several phosphatidylserine and acylphosphatidylglycerol species during egress. We thus show that, in addition to its previously demonstrated role in liver stage merozoite egress, LCAT is required to facilitate efficient egress in asexual blood stage malaria parasites. 
LB  - PUB:(DE-HGF)16
C6  - pmid:37352369
UR  - <Go to ISI:>//WOS:001016234800001
DO  - DOI:10.1371/journal.ppat.1011449
UR  - https://bib-pubdb1.desy.de/record/601556
ER  -