Journal Article

PUBDB-2023-00953

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dose-efficient Scanning Compton X-ray Microscopy

Li, T. (First author)DESY* ; Dresselhaus, J. L.CFEL* ; Ivanov, N.CFEL*XFEL.EU*DESY* ; Prasciolu, M.CFEL*XFEL.EU*DESY* ; Fleckenstein, H.CFEL*XFEL.EU*DESY* ; Yefanov, O.CFEL*XFEL.EU*DESY* ; Zhang, W.CFEL*DESY* ; Pennicard, D.CFEL*DESY* ; Dippel, A.-C.DESY* ; Gutowski, O.DESY* ; Villanueva Perez, P.Extern*XFEL.EU* ; Chapman, H. N. (Corresponding author)CFEL*XFEL.EU*DESY* ; Bajt, S. (Corresponding author)CFEL*XFEL.EU*DESY*

2023
Nature Publishing Group London

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Please use a persistent id in citations: doi:10.1038/s41377-023-01176-5  doi:10.3204/PUBDB-2023-00953

Abstract: The highest resolution of images of soft matter and biological materials is ultimately limited by modification of the structure, induced by the necessarily high energy of short-wavelength radiation. Imaging the inelastically scattered X-rays at a photon energy of 60 keV (0.02 nm wavelength) offers greater signal per energy transferred to the sample than coherent-scattering techniques such as phase-contrast microscopy and projection holography. We present images of dried, unstained, and unfixed biological objects obtained by scanning Compton X-ray microscopy, at a resolution of about 40 nm. This microscope was realised using novel wedged multilayer Laue lenses that were fabricated to sub-ångström precision, a new wavefront measurement scheme for hard X rays, and efficient pixel-array detectors. The doses required to form these images were as little as 0.02% of the tolerable dose and 0.05% of that needed for phase-contrast imaging at similar resolution using 12 keV photon energy. The images obtained provide a quantitative map of the projected mass density in the sample, as confirmed by imaging a silicon wedge. Based on these results, we find that it should be possible to obtain radiation damage-free images of biological samples at a resolution below 10 nm.

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Contributing Institute(s):

1. FS-Arbeitsgruppe (FS-ML)
2. FS-CFEL-1 (Group Leader: Henry Chapman) (CFEL-I)
3. Lund University (LUND)
4. FS-Detektor Systeme (FS-DS)
5. PETRA-D (FS-PETRA-D)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
2. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
3. DFG project 390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994) (390715994)
4. DFG project 194651731 - EXC 1074: Hamburger Zentrum für ultraschnelle Beobachtung (CUI): Struktur, Dynamik und Kontrolle von Materie auf atomarer Skala (194651731) (194651731)

Experiment(s):

1. PETRA Beamline P07 (PETRA III)

Appears in the scientific report 2023

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Database coverage:
; ; ; ; Clarivate Analytics Master Journal List ; Current Contents - Engineering, Computing and Technology ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; IF >= 15 ; JCR ; PubMed Central ; SCOPUS ; Web of Science Core Collection

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