TY - JOUR AU - Li, Tang AU - Dresselhaus, Jan Lukas AU - Ivanov, Nikolay AU - Prasciolu, Mauro AU - Fleckenstein, Holger AU - Yefanov, Oleksandr AU - Zhang, Wenhui AU - Pennicard, David AU - Dippel, Ann-Christin AU - Gutowski, Olof AU - Villanueva Perez, Pablo AU - Chapman, Henry N. AU - Bajt, Sasa TI - Dose-efficient Scanning Compton X-ray Microscopy JO - Light VL - 12 IS - 1 SN - 2047-7538 CY - London PB - Nature Publishing Group M1 - PUBDB-2023-00953 SP - 130 PY - 2023 AB - The highest resolution of images of soft matter and biological materials is ultimately limited by modification of the structure, induced by the necessarily high energy of short-wavelength radiation. Imaging the inelastically scattered X-rays at a photon energy of 60 keV (0.02 nm wavelength) offers greater signal per energy transferred to the sample than coherent-scattering techniques such as phase-contrast microscopy and projection holography. We present images of dried, unstained, and unfixed biological objects obtained by scanning Compton X-ray microscopy, at a resolution of about 40 nm. This microscope was realised using novel wedged multilayer Laue lenses that were fabricated to sub-ångström precision, a new wavefront measurement scheme for hard X rays, and efficient pixel-array detectors. The doses required to form these images were as little as 0.02 LB - PUB:(DE-HGF)16 C6 - 37248250 UR - <Go to ISI:>//WOS:000998732800001 DO - DOI:10.1038/s41377-023-01176-5 UR - https://bib-pubdb1.desy.de/record/578755 ER -