Journal Article

PUBDB-2022-03913

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Rapid and efficient room temperature serial synchrotron crystallography using the CFEL TapeDrive

Zielinski, K.Extern* ; Prester, A.Extern*EMBL* ; Andaleeb, H.Extern*XFEL.EU* ; Bui ; Yefanov, O.CFEL*XFEL.EU*DESY* ; Catapano ; Henkel, A.CFEL*Extern*XFEL.EU*EMBL*DESY* ; Wiedorn, M. O.XFEL.EU* ; Lorbeer, O. ; Crosas Ubeda, E.EMBL*DESY* ; Meyer, J.CFEL*XFEL.EU*DESY* ; Mariani, V.XFEL.EU*DESY* ; Domaracky, M.Extern*XFEL.EU* ; White, T.CFEL*XFEL.EU*DESY* ; Fleckenstein, H.CFEL*XFEL.EU*DESY* ; Sarrou, I.XFEL.EU*DESY* ; Werner, N.EMBL* ; Betzel, C.Extern* ; Rohde, H. ; Aepfelbacher, M. ; Chapman, H. N.CFEL*XFEL.EU*DESY* ; Perbandt, M.Extern*XFEL.EU* ; Steiner, R. (Corresponding author)Extern*EMBL* ; Oberthür, D. (Corresponding author)CFEL*XFEL.EU*DESY*

2022
Chester

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Please use a persistent id in citations: doi:10.1107/S2052252522010193  doi:10.3204/PUBDB-2022-03913

Abstract: Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micron sized crystals of biological macromolecules. However, it suffers from the fact that data collection is not yet as routine and takes currently significantly longer as the standard rotation series cryo-crystallography. Thus its use for high-throughput approaches, such as fragment-based drug screening, where the possibility to measure at physiological temperatures would be a great benefit, is impaired. On the way to high-throughput serial synchrotron crystallography, it is shown here, using a conveyor belt based sample delivery system – the CFEL TapeDrive – with three different proteins of biological relevance (K. pneumoniae CTX-M-14 β-lactamase, Nectria haematococca xylanase GH11 and Aspergillus flavus urate oxidase), that complete data sets can be collected in less than a minute and that only minimal amounts of sample are required.

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Contributing Institute(s):

1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)
2. Scientific computing (FS-SC)
3. Experimentebetreuung PETRA III (FS-PE)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
2. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
3. DFG project 390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994) (390715994)
4. DFG project 194651731 - EXC 1074: Hamburger Zentrum für ultraschnelle Beobachtung (CUI): Struktur, Dynamik und Kontrolle von Materie auf atomarer Skala (194651731) (194651731)

Experiment(s):

1. PETRA Beamline P11 (PETRA III)

Appears in the scientific report 2022

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