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| 001 | 480632 | ||
| 005 | 20250715175519.0 | ||
| 024 | 7 | _ | |a 10.1107/S2052252522010193 |2 doi |
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| 100 | 1 | _ | |a Zielinski, Kara |0 P:(DE-H253)PIP1030666 |b 0 |
| 245 | _ | _ | |a Rapid and efficient room temperature serial synchrotron crystallography using the CFEL TapeDrive |
| 260 | _ | _ | |a Chester |c 2022 |
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| 520 | _ | _ | |a Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micron sized crystals of biological macromolecules. However, it suffers from the fact that data collection is not yet as routine and takes currently significantly longer as the standard rotation series cryo-crystallography. Thus its use for high-throughput approaches, such as fragment-based drug screening, where the possibility to measure at physiological temperatures would be a great benefit, is impaired. On the way to high-throughput serial synchrotron crystallography, it is shown here, using a conveyor belt based sample delivery system – the CFEL TapeDrive – with three different proteins of biological relevance (K. pneumoniae CTX-M-14 β-lactamase, Nectria haematococca xylanase GH11 and Aspergillus flavus urate oxidase), that complete data sets can be collected in less than a minute and that only minimal amounts of sample are required. |
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| 700 | 1 | _ | |a Meyer, Jan |0 P:(DE-H253)PIP1016998 |b 10 |
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| 700 | 1 | _ | |a Steiner, Roberto |0 P:(DE-H253)PIP1032175 |b 22 |e Corresponding author |
| 700 | 1 | _ | |a Oberthür, Dominik |0 P:(DE-H253)PIP1012087 |b 23 |e Corresponding author |
| 773 | _ | _ | |a 10.1107/S2052252522010193 |0 PERI:(DE-600)2754953-7 |n 6 |p 778 - 791 |t IUCrJ |v 9 |y 2022 |x 2052-2525 |
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