TY  - JOUR
AU  - Zielinski, Kara
AU  - Prester, Andreas
AU  - Andaleeb, Hina
AU  - Bui
AU  - Yefanov, Oleksandr
AU  - Catapano
AU  - Henkel, Alessandra
AU  - Wiedorn, Max O.
AU  - Lorbeer, Olga
AU  - Crosas Ubeda, Eva
AU  - Meyer, Jan
AU  - Mariani, Valerio
AU  - Domaracky, Martin
AU  - White, Thomas
AU  - Fleckenstein, Holger
AU  - Sarrou, Iosifina
AU  - Werner, Nadine
AU  - Betzel, Christian
AU  - Rohde, Holger
AU  - Aepfelbacher, Martin
AU  - Chapman, Henry N.
AU  - Perbandt, Markus
AU  - Steiner, Roberto
AU  - Oberthür, Dominik
TI  - Rapid and efficient room temperature serial synchrotron crystallography using the CFEL TapeDrive
JO  - IUCrJ
VL  - 9
IS  - 6
SN  - 2052-2525
CY  - Chester
M1  - PUBDB-2022-03913
SP  - 778 - 791
PY  - 2022
AB  - Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micron sized crystals of biological macromolecules. However, it suffers from the fact that data collection is not yet as routine and takes currently significantly longer as the standard rotation series cryo-crystallography. Thus its use for high-throughput approaches, such as fragment-based drug screening, where the possibility to measure at physiological temperatures would be a great benefit, is impaired. On the way to high-throughput serial synchrotron crystallography, it is shown here, using a conveyor belt based sample delivery system – the CFEL TapeDrive – with three different proteins of biological relevance (K. pneumoniae CTX-M-14 β-lactamase, Nectria haematococca xylanase GH11 and Aspergillus flavus urate oxidase), that complete data sets can be collected in less than a minute and that only minimal amounts of sample are required.
LB  - PUB:(DE-HGF)16
C6  - pmid:36381150
UR  - <Go to ISI:>//WOS:000886786600009
DO  - DOI:10.1107/S2052252522010193
UR  - https://bib-pubdb1.desy.de/record/480632
ER  -