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@ARTICLE{Zielinski:480632,
author = {Zielinski, Kara and Prester, Andreas and Andaleeb, Hina and
Bui and Yefanov, Oleksandr and Catapano and Henkel,
Alessandra and Wiedorn, Max O. and Lorbeer, Olga and Crosas
Ubeda, Eva and Meyer, Jan and Mariani, Valerio and
Domaracky, Martin and White, Thomas and Fleckenstein, Holger
and Sarrou, Iosifina and Werner, Nadine and Betzel,
Christian and Rohde, Holger and Aepfelbacher, Martin and
Chapman, Henry N. and Perbandt, Markus and Steiner, Roberto
and Oberthür, Dominik},
title = {{R}apid and efficient room temperature serial synchrotron
crystallography using the {CFEL} {T}ape{D}rive},
journal = {IUCrJ},
volume = {9},
number = {6},
issn = {2052-2525},
address = {Chester},
reportid = {PUBDB-2022-03913},
pages = {778 - 791},
year = {2022},
abstract = {Serial crystallography at conventional synchrotron light
sources (SSX) offers the possibility to routinely collect
data at room temperature using micron sized crystals of
biological macromolecules. However, it suffers from the fact
that data collection is not yet as routine and takes
currently significantly longer as the standard rotation
series cryo-crystallography. Thus its use for
high-throughput approaches, such as fragment-based drug
screening, where the possibility to measure at physiological
temperatures would be a great benefit, is impaired. On the
way to high-throughput serial synchrotron crystallography,
it is shown here, using a conveyor belt based sample
delivery system – the CFEL TapeDrive – with three
different proteins of biological relevance (K. pneumoniae
CTX-M-14 β-lactamase, Nectria haematococca xylanase GH11
and Aspergillus flavus urate oxidase), that complete data
sets can be collected in less than a minute and that only
minimal amounts of sample are required.},
cin = {FS-CFEL-1 / FS-SC / FS-PE},
ddc = {530},
cid = {I:(DE-H253)FS-CFEL-1-20120731 / I:(DE-H253)FS-SC-20210408 /
I:(DE-H253)FS-PE-20120731},
pnm = {633 - Life Sciences – Building Blocks of Life: Structure
and Function (POF4-633) / 6G3 - PETRA III (DESY) (POF4-6G3)
/ DFG project 390715994 - EXC 2056: CUI: Advanced Imaging of
Matter (390715994) / DFG project 194651731 - EXC 1074:
Hamburger Zentrum für ultraschnelle Beobachtung (CUI):
Struktur, Dynamik und Kontrolle von Materie auf atomarer
Skala (194651731)},
pid = {G:(DE-HGF)POF4-633 / G:(DE-HGF)POF4-6G3 /
G:(GEPRIS)390715994 / G:(GEPRIS)194651731},
experiment = {EXP:(DE-H253)P-P11-20150101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:36381150},
UT = {WOS:000886786600009},
doi = {10.1107/S2052252522010193},
url = {https://bib-pubdb1.desy.de/record/480632},
}