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X-ray screening identifies active site and allosteric inhibitors of SARS-CoV-2 main protease
Günther, S. (Corresponding author)CFEL*DESY* ; Reinke, P. Y. A.CFEL*EMBL*DESY* ; Fernández-García, Y.Extern*EMBL* ; Lieske, J.CFEL*EMBL*DESY* ; Lane, T. J.CFEL*Extern*DESY* ; Ginn, H. M.CFEL*Extern*XFEL.EU* ; Koua, F. H. M. ; Ehrt, C. ; Ewert, W.CFEL*EMBL*DESY* ; Oberthuer, D.CFEL*DESY* ; Yefanov, O.CFEL*EMBL*DESY* ; Meier, S.CFEL*EMBL* ; Lorenzen, K.XFEL.EU* ; Krichel, B.Extern* ; Kopicki, J.-D.Extern* ; Gelisio, L.CFEL*DESY* ; Brehm, W.CFEL*DESY* ; Dunkel, I. ; Seychell, B.CFEL*XFEL.EU*EMBL* ; Gieseler, H.CFEL*Extern*EMBL* ; Norton-Baker, B.CFEL*MPG*EMBL* ; Escudero-Pérez, B. ; Domaracky, M.CFEL*DESY* ; Saouane, S.Extern*DESY* ; Tolstikova, A.CFEL*DESY* ; White, T. A.CFEL*DESY* ; Hänle, A.CFEL*DESY* ; Groessler, M.CFEL* ; Fleckenstein, H.CFEL*DESY* ; Trost, F.CFEL*DESY* ; Galchenkova, M.CFEL*Extern*XFEL.EU*DESY* ; Gevorkov, Y.CFEL*Extern*XFEL.EU*DESY* ; Li, C.CFEL*XFEL.EU*DESY* ; Awel, S.CFEL*DESY* ; Peck, A. ; Barthelmess, M.CFEL*DESY* ; Schluenzen, F.DESY* ; Lourdu Xavier, P. ; Werner, N.Extern*EMBL* ; Andaleeb, H.Extern*XFEL.EU*EMBL* ; Ullah, N.EMBL*DESY* ; Falke, S.Extern* ; Srinivasan, V.Extern*EMBL* ; França, B. A.XFEL.EU* ; Schwinzer, M.Extern*EMBL* ; Brognaro, H.Extern*XFEL.EU*EMBL*DESY* ; Rogers, C. ; Melo, D. ; Zaitseva-Doyle, J. J. ; Knoska, J.CFEL*XFEL.EU*DESY* ; Peña-Murillo, G. E. ; Mashhour, A. R. ; Hennicke, V.CFEL*DESY* ; Fischer, P.CFEL*DESY* ; Hakanpää, J.DESY* ; Meyer, J.DESY* ; Gribbon, P. ; Ellinger, B. ; Kuzikov, M. ; Wolf, M. ; Beccari, A. R. ; Bourenkov, G.EMBL* ; von Stetten, D.EMBL* ; Pompidor, G.EMBL*DESY* ; Bento, I. ; Panneerselvam, S.Extern*EMBL* ; Karpics, I. ; Schneider, T. R.EMBL* ; Garcia-Alai, M. M. ; Niebling, S. ; Guenther, C.CSSB*EMBL* ; Schmidt, C.XFEL.EU*EMBL* ; Schubert, R.XFEL.EU*EMBL* ; Han, H.XFEL.EU*EMBL* ; Boger, J. ; Monteiro, D. C. F.Extern*EMBL* ; Zhang, L. ; Sun, X. ; Pletzer-Zelgert, J. ; Wollenhaupt, J.Extern*EMBL* ; Feiler, C. G.Extern*EMBL* ; Weiss, M. S. ; Schulz, E.-C.CFEL*MPG* ; Mehrabi, P.CFEL*MPG*EMBL*Extern* ; Karničar, K. ; Usenik, A. ; Loboda, J. ; Tidow, H.Extern*EMBL* ; Chari, A. ; Hilgenfeld, R. ; Uetrecht, C.XFEL.EU* ; Cox, R. ; Zaliani, A. ; Beck, T.Extern*EMBL* ; Rarey, M.Extern* ; Günther, S. ; Turk, D.CFEL* ; Hinrichs, W.Extern* ; Chapman, H. N.CFEL*DESY* ; Pearson, A. R.Extern*EMBL* ; Betzel, C.Extern* ; Meents, A. (Corresponding author)CFEL*DESY*
2021
Assoc.
Washington, DC
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Please use a persistent id in citations: doi:10.1126/science.abf7945 doi:10.3204/PUBDB-2021-01692
Abstract: The coronavirus disease (COVID-19) caused by SARS-CoV-2 is creating tremendous human suffering. To date, no effective drug is available to directly treat the disease. In a search for a drug against COVID-19, we have performed a high-throughput X-ray crystallographic screen of two repurposing drug libraries against the SARS-CoV-2 main protease (Mpro), which is essential for viral replication. In contrast to commonly applied X-ray fragment screening experiments with molecules of low complexity, our screen tested already approved drugs and drugs in clinical trials. From the three-dimensional protein structures, we identified 37 compounds that bind to Mpro. In subsequent cell-based viral reduction assays, one peptidomimetic and six non-peptidic compounds showed antiviral activity at non-toxic concentrations. We identified two allosteric binding sites representing attractive targets for drug development against SARS-CoV-2.
Contributing Institute(s):
- FS-CFEL-1 (Group Leader: Henry Chapman) (CFEL-I)
- Arwen Pearson - CUI (CFEL-TRSB)
- Experimentebetreuung PETRA III (FS-PET-D)
- FS_FS-SC (FS_FS-SC)
- Informationstechnologie (IT)
- EMBL (EMBL)
- Uni Hamburg (U HH)
- Centre for Structural Systems Biology (FS-CSSB-GS)
- MPSD (CFEL-ARD)
- European XFEL Projekt Team (Eur.XFEL)
- CSSB - Leibniz-Institut für Experimentelle Virologie (LIV) / DESY - Charlotte Uetrecht (CSSB-LIV/DESY-CU)
- CSSB-CF-SPC (CSSB-CF-SPC)
Research Program(s):
- 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
- 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
- HIDSS-0002 - DASHH: Data Science in Hamburg - Helmholtz Graduate School for the Structure of Matter (2019_IVF-HIDSS-0002) (2019_IVF-HIDSS-0002)
- AIM, DFG project G:(GEPRIS)390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994) (390715994)
- EXSCALATE4CoV - EXaSCale smArt pLatform Against paThogEns for Corona Virus (101003551) (101003551)
- Ex-Net-0002-Phase2-3 - Advanced Imaging of Matter: Structure, Dynamics and Control on the Atomic Scale - AIM (2018_Ex-Net-0002-Phase2-3) (2018_Ex-Net-0002-Phase2-3)
- DFG project G:(GEPRIS)194651731 - EXC 1074: Hamburger Zentrum für ultraschnelle Beobachtung (CUI): Struktur, Dynamik und Kontrolle von Materie auf atomarer Skala (194651731) (194651731)
Experiment(s):
- PETRA Beamline P11 (PETRA III)
- PETRA Beamline P13 (PETRA III)
- PETRA Beamline P14 (PETRA III)
Appears in the scientific report
2021
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