Journal Article

PUBDB-2020-02482

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Structure-Based Identification and Functional Characterization of a Lipocalin in the Malaria Parasite Plasmodium falciparum

Burda, P.-C.CSSB* ; Crosskey, T.EMBL* ; Lauk, K.CSSB* ; Zurborg, A.CSSB* ; Söhnchen, C. ; Liffner, B.CSSB* ; Wilcke, L.CSSB* ; Pietsch, E.CSSB* ; Strauss, J.CSSB*EMBL* ; Jeffries, C. M.Extern*EMBL* ; Svergun, D. I.EMBL* ; Wilson, D. W. ; Wilmanns, M.Extern*EMBL* ; Gilberger, T.-W. (Corresponding author)CSSB*

2020
Elsevier [New York, NY]

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Please use a persistent id in citations: doi:10.1016/j.celrep.2020.107817  doi:10.3204/PUBDB-2020-02482

Abstract: Proteins of the lipocalin family are known to bind small hydrophobic ligands and are involved in various physiological processes ranging from lipid transport to oxidative stress responses. The genome of the malaria parasite Plasmodium falciparum contains a single protein PF3D7_0925900 with a lipocalin signature. Using crystallography and small-angle X-ray scattering, we show that the protein has a tetrameric structure of typical lipocalin monomers; hence we name it P. falciparum lipocalin (PfLCN). We show that PfLCN is expressed in the intraerythrocytic stages of the parasite and localizes to the parasitophorous and food vacuoles. Conditional knockdown of PfLCN impairs parasite development, which can be rescued by treatment with the radical scavenger Trolox or by temporal inhibition of hemoglobin digestion. This suggests a key function of PfLCN in counteracting oxidative stress-induced cell damage during multiplication of parasites within erythrocytes.

Note: PIF-2018-87

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Contributing Institute(s):

1. CSSB-BNI (CSSB-BNI)
2. EMBL (EMBL)
3. Centre for Structural Systems Biology (FS-CSSB-GS)
4. CSSB-BNITM-TG (CSSB-BNITM-TG)

Research Program(s):

1. 6G3 - PETRA III (POF3-622) (POF3-622)

Experiment(s):

1. PETRA Beamline P13 (PETRA III)
2. PETRA Beamline P12 (PETRA III)

Appears in the scientific report 2020

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Database coverage:
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