Journal Article

PUBDB-2019-00177

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png DNA–DOPE–gemini surfactants complexes at low surface charge density: from structure to transfection efficiency

Hubčík, L. (Corresponding author)Extern* ; Galliková, D. ; Pullmannová, P.Extern* ; Lacinová, Ľ. ; Sulová, Z. ; Hanulová, M.DESY* ; Funari, S. d. S.DESY* ; Devínsky, F. ; Uhríková, D.Extern*

2018
AEPress Bratislava

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Please use a persistent id in citations: doi:10.4149/gpb_2017042  doi:10.3204/PUBDB-2019-00177

Abstract: DNA condensation, structure and transfection efficiency of complexes formed by gemini surfactants alkane-α,ω-diyl-bis(dodecyldimethylammonium bromide)s (CnGS12, n = 3, 6 and 12 is the number of alkane spacer carbons), dioleoylphosphatidylethanolamine (CnGS12/DOPE = 0.3 mol/mol) and DNA at low surface charge density were investigated through different techniques. Small angle X-ray diffraction showed a condensed lamellar phase with marked dependence of DNA-DNA distance on (+/-) charge ratio. High ionic strength of hydrating medium screens the interaction DNA - CnGS12/DOPE and complexed DNA represented maximally ~ 45–60% of total DNA in the solution as derived from fluorescence and UV-VIS spectroscopy. The in vitro transfection efficiency of CnGS12/DOPE liposomes on mammalian HEK 293 cell line was spacer length-dependent. C12GS12/DOPE/DNA complexes exhibited the best transfection efficiency (~ 18% GFP-expressing cells relative to all viable cells) accompanied by ~ 89% cell viability.

Note: © AEPress s.r.o

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Contributing Institute(s):

1. DOOR-User (DOOR)
2. PETRA III Extension (FS-PEX)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)

Experiment(s):

1. DORIS Beamline A2 (DORIS III)

Appears in the scientific report 2018

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Database coverage:
; ; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

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