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000418090 1001_ $$0P:(DE-H253)PIP1013101$$aHubčík, Lukáš$$b0$$eCorresponding author
000418090 245__ $$aDNA–DOPE–gemini surfactants complexes at low surface charge density: from structure to transfection efficiency
000418090 260__ $$aBratislava$$bAEPress$$c2018
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000418090 520__ $$aDNA condensation, structure and transfection efficiency of complexes formed by gemini surfactants alkane-α,ω-diyl-bis(dodecyldimethylammonium bromide)s (CnGS12, n = 3, 6 and 12 is the number of alkane spacer carbons), dioleoylphosphatidylethanolamine (CnGS12/DOPE = 0.3 mol/mol) and DNA at low surface charge density were investigated through different techniques. Small angle X-ray diffraction showed a condensed lamellar phase with marked dependence of DNA-DNA distance on (+/-) charge ratio. High ionic strength of hydrating medium screens the interaction DNA - CnGS12/DOPE and complexed DNA represented maximally ~ 45–60% of total DNA in the solution as derived from fluorescence and UV-VIS spectroscopy. The in vitro transfection efficiency of CnGS12/DOPE liposomes on mammalian HEK 293 cell line was spacer length-dependent. C12GS12/DOPE/DNA complexes exhibited the best transfection efficiency (~ 18% GFP-expressing cells relative to all viable cells) accompanied by ~ 89% cell viability.
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000418090 7001_ $$aGalliková, Dominika$$b1
000418090 7001_ $$0P:(DE-H253)PIP1008449$$aPullmannová, Petra$$b2
000418090 7001_ $$aLacinová, Ľubica$$b3
000418090 7001_ $$aSulová, Zdena$$b4
000418090 7001_ $$0P:(DE-H253)PIP1003479$$aHanulová, Mária$$b5
000418090 7001_ $$0P:(DE-H253)PIP1002601$$aFunari, Sergio de Souza$$b6
000418090 7001_ $$aDevínsky, Ferdinand$$b7
000418090 7001_ $$0P:(DE-H253)PIP1008450$$aUhríková, Daniela$$b8
000418090 773__ $$0PERI:(DE-600)2053364-0$$a10.4149/gpb_2017042$$gVol. 37, no. 01, p. 57 - 69$$n1$$p57 - 69$$tGeneral physiology and biophysics$$v37$$x1338-4325$$y2018
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