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001     418090
005     20250729165644.0
024 7 _ |a 10.4149/gpb_2017042
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024 7 _ |a 1338-4325
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024 7 _ |a 10.3204/PUBDB-2019-00177
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100 1 _ |a Hubčík, Lukáš
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245 _ _ |a DNA–DOPE–gemini surfactants complexes at low surface charge density: from structure to transfection efficiency
260 _ _ |a Bratislava
|c 2018
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520 _ _ |a DNA condensation, structure and transfection efficiency of complexes formed by gemini surfactants alkane-α,ω-diyl-bis(dodecyldimethylammonium bromide)s (CnGS12, n = 3, 6 and 12 is the number of alkane spacer carbons), dioleoylphosphatidylethanolamine (CnGS12/DOPE = 0.3 mol/mol) and DNA at low surface charge density were investigated through different techniques. Small angle X-ray diffraction showed a condensed lamellar phase with marked dependence of DNA-DNA distance on (+/-) charge ratio. High ionic strength of hydrating medium screens the interaction DNA - CnGS12/DOPE and complexed DNA represented maximally ~ 45–60% of total DNA in the solution as derived from fluorescence and UV-VIS spectroscopy. The in vitro transfection efficiency of CnGS12/DOPE liposomes on mammalian HEK 293 cell line was spacer length-dependent. C12GS12/DOPE/DNA complexes exhibited the best transfection efficiency (~ 18% GFP-expressing cells relative to all viable cells) accompanied by ~ 89% cell viability.
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700 1 _ |a Galliková, Dominika
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700 1 _ |a Pullmannová, Petra
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700 1 _ |a Lacinová, Ľubica
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700 1 _ |a Sulová, Zdena
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700 1 _ |a Hanulová, Mária
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700 1 _ |a Funari, Sergio de Souza
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700 1 _ |a Devínsky, Ferdinand
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700 1 _ |a Uhríková, Daniela
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773 _ _ |a 10.4149/gpb_2017042
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