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@ARTICLE{Hubk:418090,
      author       = {Hubčík, Lukáš and Galliková, Dominika and
                      Pullmannová, Petra and Lacinová, Ľubica and Sulová,
                      Zdena and Hanulová, Mária and Funari, Sergio de Souza and
                      Devínsky, Ferdinand and Uhríková, Daniela},
      title        = {{DNA}–{DOPE}–gemini surfactants complexes at low
                      surface charge density: from structure to transfection
                      efficiency},
      journal      = {General physiology and biophysics},
      volume       = {37},
      number       = {1},
      issn         = {1338-4325},
      address      = {Bratislava},
      publisher    = {AEPress},
      reportid     = {PUBDB-2019-00177},
      pages        = {57 - 69},
      year         = {2018},
      note         = {© AEPress s.r.o},
      abstract     = {DNA condensation, structure and transfection efficiency of
                      complexes formed by gemini surfactants
                      alkane-α,ω-diyl-bis(dodecyldimethylammonium bromide)s
                      (CnGS12, n = 3, 6 and 12 is the number of alkane spacer
                      carbons), dioleoylphosphatidylethanolamine (CnGS12/DOPE =
                      0.3 mol/mol) and DNA at low surface charge density were
                      investigated through different techniques. Small angle X-ray
                      diffraction showed a condensed lamellar phase with marked
                      dependence of DNA-DNA distance on (+/-) charge ratio. High
                      ionic strength of hydrating medium screens the interaction
                      DNA - CnGS12/DOPE and complexed DNA represented maximally ~
                      $45–60\%$ of total DNA in the solution as derived from
                      fluorescence and UV-VIS spectroscopy. The in vitro
                      transfection efficiency of CnGS12/DOPE liposomes on
                      mammalian HEK 293 cell line was spacer length-dependent.
                      C12GS12/DOPE/DNA complexes exhibited the best transfection
                      efficiency (~ $18\%$ GFP-expressing cells relative to all
                      viable cells) accompanied by ~ $89\%$ cell viability.},
      cin          = {DOOR / FS-PEX},
      ddc          = {610},
      cid          = {I:(DE-H253)HAS-User-20120731 / I:(DE-H253)FS-PEX-20130206},
      pnm          = {6215 - Soft Matter, Health and Life Sciences (POF3-621)},
      pid          = {G:(DE-HGF)POF3-6215},
      experiment   = {EXP:(DE-H253)D-A2-20150101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:29424352},
      UT           = {WOS:000425206200006},
      doi          = {10.4149/gpb_2017042},
      url          = {https://bib-pubdb1.desy.de/record/418090},
}