Journal Article

PUBDB-2017-05548

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png High-speed fixed-target serial virus crystallography

Roedig, P. (First author)DESY* ; Ginn, H. (First author) ; Pakendorf, T.DESY* ; Sutton, G. ; Harlos, K. ; Walter, T. S. ; Meyer, J.DESY* ; Fischer, P.DESY* ; Duman, R.Extern* ; Vartiainen, I.Extern* ; Reime, B.DESY* ; Warmer, M.DESY* ; Brewster, A. S. ; Young, I. D. ; Michels-Clark, T. ; Sauter, N. K. ; Kotecha, A. ; Kelly, J. ; Rowlands, D. J. ; Sikorsky, M. ; Nelson, S. ; Damiani, D. S. ; Alonso-Mori, R. ; Ren, J. ; Fry, E. E. ; David, C.Extern* ; Stuart, D. I. m. ; Wagner, A.Extern* ; Meents, A. (Corresponding author)CFEL*DESY*

2017
Nature Publishing Group London [u.a.] Nature Publishing Group

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Please use a persistent id in citations: doi:10.1038/nmeth.4335

Abstract: We report a method for serial X-ray crystallography at X-ray free-electron lasers (XFELs), which allows for full use of the current 120-Hz repetition rate of the Linear Coherent Light Source (LCLS). Using a micropatterned silicon chip in combination with the high-speed Roadrunner goniometer for sample delivery, we were able to determine the crystal structures of the picornavirus bovine enterovirus 2 (BEV2) and the cytoplasmic polyhedrosis virus type 18 polyhedrin, with total data collection times of less than 14 and 10 min, respectively. Our method requires only micrograms of sample and should therefore broaden the applicability of serial femtosecond crystallography to challenging projects for which only limited sample amounts are available. By synchronizing the sample exchange to the XFEL repetition rate, our methodallows for most efficient use of the limited beam time available at XFELs and should enable a substantial increase in sample throughput at these facilities.

Note: (c) Nature America; Post referee fulltext in progress 2; Embargo 6 months from publication

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Contributing Institute(s):

1. FS-Photon Science (FS-PS)
2. Experimentebetreuung PETRA III (FS-PE)
3. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
2. VH-VI-403 - In-Situ Nano-Imaging of Biological and Chemical Processes (2015_IFV-VH-VI-403) (2015_IFV-VH-VI-403)
3. EUCALL - European Cluster of Advanced Laser Light Sources (654220) (654220)

Experiment(s):

1. Measurement at external facility

Appears in the scientific report 2017

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Database coverage:
; BIOSIS Previews ; Current Contents - Life Sciences ; Ebsco Academic Search ; IF >= 25 ; JCR ; NCBI Molecular Biology Database ; Nationallizenz ; No Authors Fulltext ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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