TY  - JOUR
AU  - Roedig, Philip
AU  - Ginn, Helen
AU  - Pakendorf, Tim
AU  - Sutton, Geoff
AU  - Harlos, Karl
AU  - Walter, Thomas S
AU  - Meyer, Jan
AU  - Fischer, Pontus
AU  - Duman, Ramona
AU  - Vartiainen, Ismo
AU  - Reime, Bernd
AU  - Warmer, Martin
AU  - Brewster, Aaron S
AU  - Young, Iris D
AU  - Michels-Clark, Tara
AU  - Sauter, Nicholas K
AU  - Kotecha, Abhay
AU  - Kelly, James
AU  - Rowlands, David J
AU  - Sikorsky, Marcin
AU  - Nelson, Silke
AU  - Damiani, Daniel S
AU  - Alonso-Mori, Roberto
AU  - Ren, Jingshan
AU  - Fry, Elizabeth E
AU  - David, Christian
AU  - Stuart, David I m
AU  - Wagner, Armin
AU  - Meents, Alke
TI  - High-speed fixed-target serial virus crystallography
JO  - Nature methods
VL  - 14
SN  - 1548-7091
CY  - London [u.a.] Nature Publishing Group
PB  - Nature Publishing Group
M1  - PUBDB-2017-05548
SP  - 805-810
PY  - 2017
N1  - (c) Nature America; Post referee fulltext in progress 2; Embargo 6 months from publication
AB  - We report a method for serial X-ray crystallography at X-ray free-electron lasers (XFELs), which allows for full use of the current 120-Hz repetition rate of the Linear Coherent Light Source (LCLS). Using a micropatterned silicon chip in combination with the high-speed Roadrunner goniometer for sample delivery, we were able to determine the crystal structures of the picornavirus bovine enterovirus 2 (BEV2) and the cytoplasmic polyhedrosis virus type 18 polyhedrin, with total data collection times of less than 14 and 10 min, respectively. Our method requires only micrograms of sample and should therefore broaden the applicability of serial femtosecond crystallography to challenging projects for which only limited sample amounts are available. By synchronizing the sample exchange to the XFEL repetition rate, our methodallows for most efficient use of the limited beam time available at XFELs and should enable a substantial increase in sample throughput at these facilities.
LB  - PUB:(DE-HGF)16
C6  - pmid:28628129
UR  - <Go to ISI:>//WOS:000406493800020
DO  - DOI:10.1038/nmeth.4335
UR  - https://bib-pubdb1.desy.de/record/328901
ER  -