% IMPORTANT: The following is UTF-8 encoded. This means that in the presence % of non-ASCII characters, it will not work with BibTeX 0.99 or older. % Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or % “biber”. @ARTICLE{Roedig:328901, author = {Roedig, Philip and Ginn, Helen and Pakendorf, Tim and Sutton, Geoff and Harlos, Karl and Walter, Thomas S and Meyer, Jan and Fischer, Pontus and Duman, Ramona and Vartiainen, Ismo and Reime, Bernd and Warmer, Martin and Brewster, Aaron S and Young, Iris D and Michels-Clark, Tara and Sauter, Nicholas K and Kotecha, Abhay and Kelly, James and Rowlands, David J and Sikorsky, Marcin and Nelson, Silke and Damiani, Daniel S and Alonso-Mori, Roberto and Ren, Jingshan and Fry, Elizabeth E and David, Christian and Stuart, David I m and Wagner, Armin and Meents, Alke}, title = {{H}igh-speed fixed-target serial virus crystallography}, journal = {Nature methods}, volume = {14}, issn = {1548-7091}, address = {London [u.a.] Nature Publishing Group}, publisher = {Nature Publishing Group}, reportid = {PUBDB-2017-05548}, pages = {805-810}, year = {2017}, note = {(c) Nature America; Post referee fulltext in progress 2; Embargo 6 months from publication}, abstract = {We report a method for serial X-ray crystallography at X-ray free-electron lasers (XFELs), which allows for full use of the current 120-Hz repetition rate of the Linear Coherent Light Source (LCLS). Using a micropatterned silicon chip in combination with the high-speed Roadrunner goniometer for sample delivery, we were able to determine the crystal structures of the picornavirus bovine enterovirus 2 (BEV2) and the cytoplasmic polyhedrosis virus type 18 polyhedrin, with total data collection times of less than 14 and 10 min, respectively. Our method requires only micrograms of sample and should therefore broaden the applicability of serial femtosecond crystallography to challenging projects for which only limited sample amounts are available. By synchronizing the sample exchange to the XFEL repetition rate, our methodallows for most efficient use of the limited beam time available at XFELs and should enable a substantial increase in sample throughput at these facilities.}, cin = {FS-PS / FS-PE / FS-CFEL-1}, ddc = {570}, cid = {I:(DE-H253)FS-PS-20131107 / I:(DE-H253)FS-PE-20120731 / I:(DE-H253)FS-CFEL-1-20120731}, pnm = {6215 - Soft Matter, Health and Life Sciences (POF3-621) / VH-VI-403 - In-Situ Nano-Imaging of Biological and Chemical Processes $(2015_IFV-VH-VI-403)$ / EUCALL - European Cluster of Advanced Laser Light Sources (654220)}, pid = {G:(DE-HGF)POF3-6215 / $G:(DE-HGF)2015_IFV-VH-VI-403$ / G:(EU-Grant)654220}, experiment = {EXP:(DE-MLZ)External-20140101}, typ = {PUB:(DE-HGF)16}, pubmed = {pmid:28628129}, UT = {WOS:000406493800020}, doi = {10.1038/nmeth.4335}, url = {https://bib-pubdb1.desy.de/record/328901}, }