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000328901 1001_ $$0P:(DE-H253)PIP1018353$$aRoedig, Philip$$b0$$eFirst author
000328901 245__ $$aHigh-speed fixed-target serial virus crystallography
000328901 260__ $$aLondon [u.a.] Nature Publishing Group$$bNature Publishing Group$$c2017
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000328901 520__ $$aWe report a method for serial X-ray crystallography at X-ray free-electron lasers (XFELs), which allows for full use of the current 120-Hz repetition rate of the Linear Coherent Light Source (LCLS). Using a micropatterned silicon chip in combination with the high-speed Roadrunner goniometer for sample delivery, we were able to determine the crystal structures of the picornavirus bovine enterovirus 2 (BEV2) and the cytoplasmic polyhedrosis virus type 18 polyhedrin, with total data collection times of less than 14 and 10 min, respectively. Our method requires only micrograms of sample and should therefore broaden the applicability of serial femtosecond crystallography to challenging projects for which only limited sample amounts are available. By synchronizing the sample exchange to the XFEL repetition rate, our methodallows for most efficient use of the limited beam time available at XFELs and should enable a substantial increase in sample throughput at these facilities.
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000328901 7001_ $$aSutton, Geoff$$b3
000328901 7001_ $$aHarlos, Karl$$b4
000328901 7001_ $$aWalter, Thomas S$$b5
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000328901 7001_ $$aBrewster, Aaron S$$b12
000328901 7001_ $$0P:(DE-HGF)0$$aYoung, Iris D$$b13
000328901 7001_ $$aMichels-Clark, Tara$$b14
000328901 7001_ $$aSauter, Nicholas K$$b15
000328901 7001_ $$aKotecha, Abhay$$b16
000328901 7001_ $$aKelly, James$$b17
000328901 7001_ $$aRowlands, David J$$b18
000328901 7001_ $$aSikorsky, Marcin$$b19
000328901 7001_ $$aNelson, Silke$$b20
000328901 7001_ $$aDamiani, Daniel S$$b21
000328901 7001_ $$0P:(DE-HGF)0$$aAlonso-Mori, Roberto$$b22
000328901 7001_ $$aRen, Jingshan$$b23
000328901 7001_ $$aFry, Elizabeth E$$b24
000328901 7001_ $$0P:(DE-H253)PIP1013976$$aDavid, Christian$$b25
000328901 7001_ $$0P:(DE-HGF)0$$aStuart, David I m$$b26
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000328901 7001_ $$0P:(DE-H253)PIP1006208$$aMeents, Alke$$b28$$eCorresponding author
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