A data set from flash X-ray imaging of carboxysomes

Hantke, Max F.; Andreasson, Jakob; Schorb, Sebastian; Maia, Filipe R. N. C.; Loh, Duane; Stellato, Francesco; Hajdu, Janos; Rudenko, Artem; Barty, Anton; Foucar, Lutz; Bozek, John D.; Kirian, Richard A.; Bari, Sadia; Timneanu, Nicusor; Svenda, Martin; Rolles, Daniel; Hasse, Dirk; Epp, Sascha; Chapman, Henry N.; Bostedt, Christoph; Mühlig, Kerstin; Ekeberg, Tomas; Iwan, Bianca; van der Schot, Gijs; DePonte, Daniel P.; John, Katja; Uetrecht, Charlotte; Carron, Sebastian; Martin, Andrew V.; Kimmel, Nils; Ingelman, Margareta; Westphal, Daniel; Hartmann, Robert; Carlsson, Gunilla H.; Ferguson, Ken; Bielecki, Johan; Andersson, Inger; Liang, Mengning; Seibert, M. Marvin; Larsson, Daniel S. D.

doi:10.1038/sdata.2016.61

Journal Article

PUBDB-2016-04849

A data set from flash X-ray imaging of carboxysomes

Hantke, M. F. ; Hasse, D. ; Ekeberg, T.CFEL*DESY* ; John, K. ; Svenda, M. ; Loh, D.CFEL*DESY* ; Martin, A. V. ; Timneanu, N. ; Larsson, D. S. D. ; van der Schot, G. ; Carlsson, G. H. ; Ingelman, M. ; Andreasson, J. ; Westphal, D. ; Iwan, B. ; Uetrecht, C.XFEL.EU* ; Bielecki, J.XFEL.EU* ; Liang, M.CFEL*DESY* ; Stellato, F.CFEL*DESY* ; DePonte, D. P.CFEL*DESY* ; Bari, S.DESY* ; Hartmann, R. ; Kimmel, N. ; Kirian, R. A. ; Seibert, M. M. ; Mühlig, K. ; Schorb, S. ; Ferguson, K. ; Bostedt, C.Extern* ; Carron, S. ; Bozek, J. D. ; Rolles, D. ; Rudenko, A. ; Foucar, L. ; Epp, S.CFEL*MPG* ; Chapman, H. N.CFEL*DESY* ; Barty, A. ; Andersson, I. ; Hajdu, J. ; Maia, F. R. N. C. (Corresponding author)

2016
Nature Publ. Group London

Scientific data 3, 160061 (2016) [10.1038/sdata.2016.61]

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Please use a persistent id in citations: doi:10.1038/sdata.2016.61

Abstract: Ultra-intense femtosecond X-ray pulses from X-ray lasers permit structural studies on single particles and biomolecules without crystals. We present a large data set on inherently heterogeneous, polyhedral carboxysome particles. Carboxysomes are cell organelles that vary in size and facilitate up to 40% of Earth’s carbon fixation by cyanobacteria and certain proteobacteria. Variation in size hinders crystallization. Carboxysomes appear icosahedral in the electron microscope. A protein shell encapsulates a large number of Rubisco molecules in paracrystalline arrays inside the organelle. We used carboxysomes with a mean diameter of 115±26 nm from Halothiobacillus neapolitanus. A new aerosol sample-injector allowed us to record 70,000 low-noise diffraction patterns in 12 min. Every diffraction pattern is a unique structure measurement and high-throughput imaging allows sampling the space of structural variability. The different structures can be separated and phased directly from the diffraction data and open a way for accurate, high-throughput studies on structures and structural heterogeneity in biology and elsewhere.

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