000311539 001__ 311539
000311539 005__ 20250717104714.0
000311539 0247_ $$2doi$$a10.1038/sdata.2016.61
000311539 0247_ $$2WOS$$aWOS:000390225400006
000311539 0247_ $$2pmid$$apmid:27479842
000311539 0247_ $$2openalex$$aopenalex:W2487826660
000311539 037__ $$aPUBDB-2016-04849
000311539 041__ $$aEnglish
000311539 082__ $$a610
000311539 1001_ $$aHantke, Max F.$$b0
000311539 245__ $$aA data set from flash X-ray imaging of carboxysomes
000311539 260__ $$aLondon$$bNature Publ. Group$$c2016
000311539 3367_ $$2DRIVER$$aarticle
000311539 3367_ $$2DataCite$$aOutput Types/Journal article
000311539 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1479898197_27378
000311539 3367_ $$2BibTeX$$aARTICLE
000311539 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000311539 3367_ $$00$$2EndNote$$aJournal Article
000311539 520__ $$aUltra-intense femtosecond X-ray pulses from X-ray lasers permit structural studies on single particles and biomolecules without crystals. We present a large data set on inherently heterogeneous, polyhedral carboxysome particles. Carboxysomes are cell organelles that vary in size and facilitate up to 40% of Earth’s carbon fixation by cyanobacteria and certain proteobacteria. Variation in size hinders crystallization. Carboxysomes appear icosahedral in the electron microscope. A protein shell encapsulates a large number of Rubisco molecules in paracrystalline arrays inside the organelle. We used carboxysomes with a mean diameter of 115±26 nm from Halothiobacillus neapolitanus. A new aerosol sample-injector allowed us to record 70,000 low-noise diffraction patterns in 12 min. Every diffraction pattern is a unique structure measurement and high-throughput imaging allows sampling the space of structural variability. The different structures can be separated and phased directly from the diffraction data and open a way for accurate, high-throughput studies on structures and structural heterogeneity in biology and elsewhere.
000311539 536__ $$0G:(DE-HGF)POF3-6215$$a6215 - Soft Matter, Health and Life Sciences (POF3-621)$$cPOF3-621$$fPOF III$$x0
000311539 588__ $$aDataset connected to CrossRef
000311539 693__ $$0EXP:(DE-MLZ)External-20140101$$5EXP:(DE-MLZ)External-20140101$$eMeasurement at external facility$$x0
000311539 7001_ $$aHasse, Dirk$$b1
000311539 7001_ $$0P:(DE-H253)PIP1007725$$aEkeberg, Tomas$$b2
000311539 7001_ $$aJohn, Katja$$b3
000311539 7001_ $$aSvenda, Martin$$b4
000311539 7001_ $$0P:(DE-H253)PIP1013332$$aLoh, Duane$$b5
000311539 7001_ $$aMartin, Andrew V.$$b6
000311539 7001_ $$aTimneanu, Nicusor$$b7
000311539 7001_ $$aLarsson, Daniel S. D.$$b8
000311539 7001_ $$avan der Schot, Gijs$$b9
000311539 7001_ $$aCarlsson, Gunilla H.$$b10
000311539 7001_ $$aIngelman, Margareta$$b11
000311539 7001_ $$aAndreasson, Jakob$$b12
000311539 7001_ $$aWestphal, Daniel$$b13
000311539 7001_ $$aIwan, Bianca$$b14
000311539 7001_ $$0P:(DE-H253)PIP1014042$$aUetrecht, Charlotte$$b15
000311539 7001_ $$0P:(DE-H253)PIP1020945$$aBielecki, Johan$$b16
000311539 7001_ $$0P:(DE-H253)PIP1007507$$aLiang, Mengning$$b17
000311539 7001_ $$0P:(DE-H253)PIP1011192$$aStellato, Francesco$$b18
000311539 7001_ $$0P:(DE-H253)PIP1008249$$aDePonte, Daniel P.$$b19
000311539 7001_ $$0P:(DE-H253)PIP1014119$$aBari, Sadia$$b20
000311539 7001_ $$aHartmann, Robert$$b21
000311539 7001_ $$aKimmel, Nils$$b22
000311539 7001_ $$aKirian, Richard A.$$b23
000311539 7001_ $$aSeibert, M. Marvin$$b24
000311539 7001_ $$aMühlig, Kerstin$$b25
000311539 7001_ $$aSchorb, Sebastian$$b26
000311539 7001_ $$aFerguson, Ken$$b27
000311539 7001_ $$0P:(DE-H253)PIP1007475$$aBostedt, Christoph$$b28
000311539 7001_ $$aCarron, Sebastian$$b29
000311539 7001_ $$aBozek, John D.$$b30
000311539 7001_ $$aRolles, Daniel$$b31
000311539 7001_ $$aRudenko, Artem$$b32
000311539 7001_ $$aFoucar, Lutz$$b33
000311539 7001_ $$0P:(DE-H253)PIP1008052$$aEpp, Sascha$$b34
000311539 7001_ $$0P:(DE-H253)PIP1006324$$aChapman, Henry N.$$b35
000311539 7001_ $$aBarty, Anton$$b36
000311539 7001_ $$aAndersson, Inger$$b37
000311539 7001_ $$aHajdu, Janos$$b38
000311539 7001_ $$0P:(DE-HGF)0$$aMaia, Filipe R. N. C.$$b39$$eCorresponding author
000311539 773__ $$0PERI:(DE-600)2775191-0$$a10.1038/sdata.2016.61$$gVol. 3, p. 160061 -$$p160061 $$tScientific data$$v3$$x2052-4463$$y2016
000311539 8564_ $$uhttps://bib-pubdb1.desy.de/record/311539/files/sdata201661.pdf$$yOpenAccess
000311539 8564_ $$uhttps://bib-pubdb1.desy.de/record/311539/files/sdata201661.gif?subformat=icon$$xicon$$yOpenAccess
000311539 8564_ $$uhttps://bib-pubdb1.desy.de/record/311539/files/sdata201661.jpg?subformat=icon-1440$$xicon-1440$$yOpenAccess
000311539 8564_ $$uhttps://bib-pubdb1.desy.de/record/311539/files/sdata201661.jpg?subformat=icon-180$$xicon-180$$yOpenAccess
000311539 8564_ $$uhttps://bib-pubdb1.desy.de/record/311539/files/sdata201661.jpg?subformat=icon-640$$xicon-640$$yOpenAccess
000311539 8564_ $$uhttps://bib-pubdb1.desy.de/record/311539/files/sdata201661.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000311539 909CO $$ooai:bib-pubdb1.desy.de:311539$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000311539 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1007725$$aDeutsches Elektronen-Synchrotron$$b2$$kDESY
000311539 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1007725$$aCentre for Free-Electron Laser Science$$b2$$kCFEL
000311539 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1013332$$aDeutsches Elektronen-Synchrotron$$b5$$kDESY
000311539 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1013332$$aCentre for Free-Electron Laser Science$$b5$$kCFEL
000311539 9101_ $$0I:(DE-588)1043621512$$6P:(DE-H253)PIP1014042$$aEuropean XFEL$$b15$$kXFEL.EU
000311539 9101_ $$0I:(DE-588)1043621512$$6P:(DE-H253)PIP1020945$$aEuropean XFEL$$b16$$kXFEL.EU
000311539 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1007507$$aDeutsches Elektronen-Synchrotron$$b17$$kDESY
000311539 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1007507$$aCentre for Free-Electron Laser Science$$b17$$kCFEL
000311539 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1011192$$aDeutsches Elektronen-Synchrotron$$b18$$kDESY
000311539 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1011192$$aCentre for Free-Electron Laser Science$$b18$$kCFEL
000311539 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1008249$$aDeutsches Elektronen-Synchrotron$$b19$$kDESY
000311539 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1008249$$aCentre for Free-Electron Laser Science$$b19$$kCFEL
000311539 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1014119$$aDeutsches Elektronen-Synchrotron$$b20$$kDESY
000311539 9101_ $$0I:(DE-HGF)0$$6P:(DE-H253)PIP1007475$$aExternes Institut$$b28$$kExtern
000311539 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1008052$$aCentre for Free-Electron Laser Science$$b34$$kCFEL
000311539 9101_ $$0I:(DE-588b)2019024-4$$6P:(DE-H253)PIP1008052$$aMax-Planck-Gesellschaft zur Förderung der Wissenschaften$$b34$$kMPG
000311539 9101_ $$0I:(DE-588b)2008985-5$$6P:(DE-H253)PIP1006324$$aDeutsches Elektronen-Synchrotron$$b35$$kDESY
000311539 9101_ $$0I:(DE-H253)_CFEL-20120731$$6P:(DE-H253)PIP1006324$$aCentre for Free-Electron Laser Science$$b35$$kCFEL
000311539 9131_ $$0G:(DE-HGF)POF3-621$$1G:(DE-HGF)POF3-620$$2G:(DE-HGF)POF3-600$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$9G:(DE-HGF)POF3-6215$$aDE-HGF$$bForschungsbereich Materie$$lVon Materie zu Materialien und Leben$$vIn-house research on the structure, dynamics and function of matter$$x0
000311539 9141_ $$y2016
000311539 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000311539 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal
000311539 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ
000311539 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000311539 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000311539 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000311539 9201_ $$0I:(DE-H253)FS-SCS-20131031$$kFS-SCS$$lStrukturdynamik Chemischer Systeme$$x0
000311539 9201_ $$0I:(DE-H253)ASU-20151130$$kASU$$lbeauftragt von FS-CFEL-1$$x1
000311539 9201_ $$0I:(DE-H253)FS-CFEL-1-20120731$$kFS-CFEL-1$$lCFEL-Coherent X-Ray Imaging$$x2
000311539 9201_ $$0I:(DE-H253)Eur_XFEL-20120731$$kEur.XFEL$$lEuropean XFEL$$x3
000311539 980__ $$ajournal
000311539 980__ $$aVDB
000311539 980__ $$aUNRESTRICTED
000311539 980__ $$aI:(DE-H253)FS-SCS-20131031
000311539 980__ $$aI:(DE-H253)ASU-20151130
000311539 980__ $$aI:(DE-H253)FS-CFEL-1-20120731
000311539 980__ $$aI:(DE-H253)Eur_XFEL-20120731
000311539 9801_ $$aFullTexts