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| 001 | 311539 | ||
| 005 | 20250717104714.0 | ||
| 024 | 7 | _ | |a 10.1038/sdata.2016.61 |2 doi |
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| 037 | _ | _ | |a PUBDB-2016-04849 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 610 |
| 100 | 1 | _ | |a Hantke, Max F. |b 0 |
| 245 | _ | _ | |a A data set from flash X-ray imaging of carboxysomes |
| 260 | _ | _ | |a London |c 2016 |b Nature Publ. Group |
| 336 | 7 | _ | |a article |2 DRIVER |
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| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1479898197_27378 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
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| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Ultra-intense femtosecond X-ray pulses from X-ray lasers permit structural studies on single particles and biomolecules without crystals. We present a large data set on inherently heterogeneous, polyhedral carboxysome particles. Carboxysomes are cell organelles that vary in size and facilitate up to 40% of Earth’s carbon fixation by cyanobacteria and certain proteobacteria. Variation in size hinders crystallization. Carboxysomes appear icosahedral in the electron microscope. A protein shell encapsulates a large number of Rubisco molecules in paracrystalline arrays inside the organelle. We used carboxysomes with a mean diameter of 115±26 nm from Halothiobacillus neapolitanus. A new aerosol sample-injector allowed us to record 70,000 low-noise diffraction patterns in 12 min. Every diffraction pattern is a unique structure measurement and high-throughput imaging allows sampling the space of structural variability. The different structures can be separated and phased directly from the diffraction data and open a way for accurate, high-throughput studies on structures and structural heterogeneity in biology and elsewhere. |
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| 700 | 1 | _ | |a Hasse, Dirk |b 1 |
| 700 | 1 | _ | |a Ekeberg, Tomas |0 P:(DE-H253)PIP1007725 |b 2 |
| 700 | 1 | _ | |a John, Katja |b 3 |
| 700 | 1 | _ | |a Svenda, Martin |b 4 |
| 700 | 1 | _ | |a Loh, Duane |0 P:(DE-H253)PIP1013332 |b 5 |
| 700 | 1 | _ | |a Martin, Andrew V. |b 6 |
| 700 | 1 | _ | |a Timneanu, Nicusor |b 7 |
| 700 | 1 | _ | |a Larsson, Daniel S. D. |b 8 |
| 700 | 1 | _ | |a van der Schot, Gijs |b 9 |
| 700 | 1 | _ | |a Carlsson, Gunilla H. |b 10 |
| 700 | 1 | _ | |a Ingelman, Margareta |b 11 |
| 700 | 1 | _ | |a Andreasson, Jakob |b 12 |
| 700 | 1 | _ | |a Westphal, Daniel |b 13 |
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| 700 | 1 | _ | |a Bari, Sadia |0 P:(DE-H253)PIP1014119 |b 20 |
| 700 | 1 | _ | |a Hartmann, Robert |b 21 |
| 700 | 1 | _ | |a Kimmel, Nils |b 22 |
| 700 | 1 | _ | |a Kirian, Richard A. |b 23 |
| 700 | 1 | _ | |a Seibert, M. Marvin |b 24 |
| 700 | 1 | _ | |a Mühlig, Kerstin |b 25 |
| 700 | 1 | _ | |a Schorb, Sebastian |b 26 |
| 700 | 1 | _ | |a Ferguson, Ken |b 27 |
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| 700 | 1 | _ | |a Carron, Sebastian |b 29 |
| 700 | 1 | _ | |a Bozek, John D. |b 30 |
| 700 | 1 | _ | |a Rolles, Daniel |b 31 |
| 700 | 1 | _ | |a Rudenko, Artem |b 32 |
| 700 | 1 | _ | |a Foucar, Lutz |b 33 |
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| 700 | 1 | _ | |a Chapman, Henry N. |0 P:(DE-H253)PIP1006324 |b 35 |
| 700 | 1 | _ | |a Barty, Anton |b 36 |
| 700 | 1 | _ | |a Andersson, Inger |b 37 |
| 700 | 1 | _ | |a Hajdu, Janos |b 38 |
| 700 | 1 | _ | |a Maia, Filipe R. N. C. |0 P:(DE-HGF)0 |b 39 |e Corresponding author |
| 773 | _ | _ | |a 10.1038/sdata.2016.61 |g Vol. 3, p. 160061 - |0 PERI:(DE-600)2775191-0 |p 160061 |t Scientific data |v 3 |y 2016 |x 2052-4463 |
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