Journal Article

PUBDB-2015-04279

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Towards phasing using high X-ray intensity

Galli, L.CFEL*DESY* ; Son, S.-K.CFEL*DESY* ; Barends, T. R. M. ; White, T.CFEL*>Extern*DESY* ; Barty, A.CFEL*DESY* ; Botha, S.>Extern* ; Boutet, S.>Extern* ; Caleman, C.CFEL*DESY* ; Doak, R. B.>Extern* ; Nanao, M. H. ; Nass, K.CFEL*DESY* ; Shoeman, R. L. ; Timneanu, N.>Extern* ; Santra, R.CFEL*DESY* ; Schlichting, I.>Extern* ; Chapman, H. N. (Corresponding author)CFEL*DESY*

2015
International Union of Crystallography (IUCr) Chester

This record in other databases:        

Please use a persistent id in citations: doi:10.1107/S2052252515014049

Abstract: X-ray free-electron lasers (XFELs) show great promise for macromolecular structure determination from sub-micrometre-sized crystals, using the emerging method of serial femtosecond crystallography. The extreme brightness of the XFEL radiation can multiply ionize most, if not all, atoms in a protein, causing their scattering factors to change during the pulse, with a preferential ‘bleaching’ of heavy atoms. This paper investigates the effects of electronic damage on experimental data collected from a Gd derivative of lysozyme microcrystals at different X-ray intensities, and the degree of ionization of Gd atoms is quantified from phased difference Fourier maps. A pattern sorting scheme isproposed to maximize the ionization contrast and the way in which the local electronic damage can be used for a new experimental phasing method is discussed.

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Contributing Institute(s):

1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)
2. CFEL-Theory (FS-CFEL-3)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
2. 05E13GU1 - Entwicklung eines "Serial Femtosecond Crystallography (SFX)"-Messtandes am Europäischen Elektronenlaser XFEL (BMBF-05E13GU1) (BMBF-05E13GU1)

Experiment(s):

1. Experiments at CFEL

Appears in the scientific report 2015

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Database coverage:
; ; ; ; Current Contents - Physical, Chemical and Earth Sciences ; NCBI Molecular Biology Database ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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