Journal Article

PUBDB-2015-01346

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Lipidic Cubic Phase Serial Millisecond Crystallography using Synchrotron Radiation

Nogly, P. (Corresponding Author) ; James, D. ; Wang, D. ; White, T. A.CFEL* ; Zatsepin, N. ; Shilova, A. ; Nelson, G. ; Liu, H. ; Johansson, L. ; Heymann, M.CFEL* ; Jaeger, K. ; Metz, M.CFEL*>Extern* ; Wickstrand, C. ; Wu, W. ; Båth, P. ; Berntsen, P. ; Oberthuer, D.CFEL*>Extern* ; Panneels, V. ; Cherezov, V. ; Chapman, H.CFEL*DESY* ; Schertler, G. ; Neutze, R. ; Spence, J. ; Moraes, I. ; Burghammer, M. ; Standfuss, J. ; Weierstall, U.>Extern*

2015
International Union of Crystallography (IUCr) Chester

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Please use a persistent id in citations: doi:10.1107/S2052252514026487

Abstract: Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins.Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs). Here, the adaptation of this technology to perform serial millisecond crystallography (SMX) at more widely available synchrotron microfocus beamlines is described. Compared with conventional microcrystallography, LCP-SMX eliminates the need for difficult handling of individual crystals and allows for data collection at room temperature. The technology is demonstrated by solving a structure of the light-driven protonpump bacteriorhodopsin (bR) at a resolution of 2.4 A ° . The room-temperature structure of bR is very similar to previous cryogenic structures but shows small yet distinct differences in the retinal ligand and proton-transfer pathway.

Note: PIF-2015-46

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Contributing Institute(s):

1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)

Research Program(s):

1. 6215 - Soft Matter, Health and Life Sciences (POF3-621) (POF3-621)
2. NANOMEM - Membrane Protein Nanocrystallography (317079) (317079)

Experiment(s):

1. Experiments at CFEL

Appears in the scientific report 2015

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Database coverage:
; ; ; ; NCBI Molecular Biology Database

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