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@ARTICLE{Nogly:207438,
author = {Nogly, Przemyslaw and James, Daniel and Wang, Dingjie and
White, Thomas A. and Zatsepin, Nadia and Shilova, Anastasya
and Nelson, Garrett and Liu, Haiguang and Johansson, Linda
and Heymann, Michael and Jaeger, Kathrin and Metz, Markus
and Wickstrand, Cecilia and Wu, Wenting and Båth, Petra and
Berntsen, Peter and Oberthuer, Dominik and Panneels, Valerie
and Cherezov, Vadim and Chapman, Henry and Schertler,
Gebhard and Neutze, Richard and Spence, John and Moraes,
Isabel and Burghammer, Manfred and Standfuss, Joerg and
Weierstall, Uwe},
title = {{L}ipidic {C}ubic {P}hase {S}erial {M}illisecond
{C}rystallography using {S}ynchrotron {R}adiation},
journal = {IUCrJ},
volume = {2},
number = {2},
issn = {2052-2525},
address = {Chester},
publisher = {International Union of Crystallography (IUCr)},
reportid = {PUBDB-2015-01346},
pages = {168 - 176},
year = {2015},
note = {PIF-2015-46},
abstract = {Lipidic cubic phases (LCPs) have emerged as successful
matrixes for the crystallization of membrane
proteins.Moreover, the viscous LCP also provides a highly
effective delivery medium for serial femtosecond
crystallography (SFX) at X-ray free-electron lasers (XFELs).
Here, the adaptation of this technology to perform serial
millisecond crystallography (SMX) at more widely available
synchrotron microfocus beamlines is described. Compared with
conventional microcrystallography, LCP-SMX eliminates the
need for difficult handling of individual crystals and
allows for data collection at room temperature. The
technology is demonstrated by solving a structure of the
light-driven protonpump bacteriorhodopsin (bR) at a
resolution of 2.4 A ° . The room-temperature structure of
bR is very similar to previous cryogenic structures but
shows small yet distinct differences in the retinal ligand
and proton-transfer pathway.},
cin = {FS-CFEL-1},
ddc = {530},
cid = {I:(DE-H253)FS-CFEL-1-20120731},
pnm = {6215 - Soft Matter, Health and Life Sciences (POF3-621) /
NANOMEM - Membrane Protein Nanocrystallography (317079)},
pid = {G:(DE-HGF)POF3-6215 / G:(EU-Grant)317079},
experiment = {EXP:(DE-H253)CFEL-Exp-20150101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000356866400007},
pubmed = {pmid:25866654},
doi = {10.1107/S2052252514026487},
url = {https://bib-pubdb1.desy.de/record/207438},
}