TY  - JOUR
AU  - Nogly, Przemyslaw
AU  - James, Daniel
AU  - Wang, Dingjie
AU  - White, Thomas A.
AU  - Zatsepin, Nadia
AU  - Shilova, Anastasya
AU  - Nelson, Garrett
AU  - Liu, Haiguang
AU  - Johansson, Linda
AU  - Heymann, Michael
AU  - Jaeger, Kathrin
AU  - Metz, Markus
AU  - Wickstrand, Cecilia
AU  - Wu, Wenting
AU  - Båth, Petra
AU  - Berntsen, Peter
AU  - Oberthuer, Dominik
AU  - Panneels, Valerie
AU  - Cherezov, Vadim
AU  - Chapman, Henry
AU  - Schertler, Gebhard
AU  - Neutze, Richard
AU  - Spence, John
AU  - Moraes, Isabel
AU  - Burghammer, Manfred
AU  - Standfuss, Joerg
AU  - Weierstall, Uwe
TI  - Lipidic Cubic Phase Serial Millisecond Crystallography using Synchrotron Radiation
JO  - IUCrJ
VL  - 2
IS  - 2
SN  - 2052-2525
CY  - Chester
PB  - International Union of Crystallography (IUCr)
M1  - PUBDB-2015-01346
SP  - 168 - 176
PY  - 2015
N1  - PIF-2015-46
AB  - Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins.Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs). Here, the adaptation of this technology to perform serial millisecond crystallography (SMX) at more widely available synchrotron microfocus beamlines is described. Compared with conventional microcrystallography, LCP-SMX eliminates the need for difficult handling of individual crystals and allows for data collection at room temperature. The technology is demonstrated by solving a structure of the light-driven protonpump bacteriorhodopsin (bR) at a resolution of 2.4 A ° . The room-temperature structure of bR is very similar to previous cryogenic structures but shows small yet distinct differences in the retinal ligand and proton-transfer pathway.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000356866400007
C6  - pmid:25866654
DO  - DOI:10.1107/S2052252514026487
UR  - https://bib-pubdb1.desy.de/record/207438
ER  -