%0 Journal Article
%A Nogly, Przemyslaw
%A James, Daniel
%A Wang, Dingjie
%A White, Thomas A.
%A Zatsepin, Nadia
%A Shilova, Anastasya
%A Nelson, Garrett
%A Liu, Haiguang
%A Johansson, Linda
%A Heymann, Michael
%A Jaeger, Kathrin
%A Metz, Markus
%A Wickstrand, Cecilia
%A Wu, Wenting
%A Båth, Petra
%A Berntsen, Peter
%A Oberthuer, Dominik
%A Panneels, Valerie
%A Cherezov, Vadim
%A Chapman, Henry
%A Schertler, Gebhard
%A Neutze, Richard
%A Spence, John
%A Moraes, Isabel
%A Burghammer, Manfred
%A Standfuss, Joerg
%A Weierstall, Uwe
%T Lipidic Cubic Phase Serial Millisecond Crystallography using Synchrotron Radiation
%J IUCrJ
%V 2
%N 2
%@ 2052-2525
%C Chester
%I International Union of Crystallography (IUCr)
%M PUBDB-2015-01346
%P 168 - 176
%D 2015
%Z PIF-2015-46
%X Lipidic cubic phases (LCPs) have emerged as successful matrixes for the crystallization of membrane proteins.Moreover, the viscous LCP also provides a highly effective delivery medium for serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs). Here, the adaptation of this technology to perform serial millisecond crystallography (SMX) at more widely available synchrotron microfocus beamlines is described. Compared with conventional microcrystallography, LCP-SMX eliminates the need for difficult handling of individual crystals and allows for data collection at room temperature. The technology is demonstrated by solving a structure of the light-driven protonpump bacteriorhodopsin (bR) at a resolution of 2.4 A ° . The room-temperature structure of bR is very similar to previous cryogenic structures but shows small yet distinct differences in the retinal ligand and proton-transfer pathway.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000356866400007
%$ pmid:25866654
%R 10.1107/S2052252514026487
%U https://bib-pubdb1.desy.de/record/207438