Journal Article

PUBDB-2025-03100

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png ChiraKit: an online tool for the analysis of circular dichroism spectroscopy data

Burastero, O. (Corresponding author)Extern*CSSB*EMBL* ; Jones, N. C. ; Defelipe, L. A. ; Zavrtanik, U. ; Hadži, S. ; Hoffmann, S. V. ; Garcia-Alai, M. M. (Corresponding author)

2025
Oxford Univ. Press Oxford

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Please use a persistent id in citations: doi:10.1093/nar/gkaf350  doi:10.3204/PUBDB-2025-03100

Abstract: Circular dichroism (CD) spectroscopy is an established biophysical technique to study chiral molecules. CD allows investigating conformational changes under varying experimental conditions and has been used to understand secondary structure, folding, and binding of proteins and nucleic acids. Here, we present ChiraKit, a user-friendly, online, and open-source tool to process raw CD data and perform advanced analysis. ChiraKit features include the calculation of protein secondary structure with the SELCON3 and SESCA algorithms, estimation of peptide helicity using the helix-ensemble model, the fitting of thermal/chemical unfolding or user-defined models, and the decomposition of spectra through singular value decomposition or principal component analysis. ChiraKit can be accessed at https://spc.embl-hamburg.de/.

Note: ISSN 1362-4962 not unique: **2 hits**.

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Contributing Institute(s):

1. CSSB-CF-SPC (CSSB-CF-SPC)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)

Experiment(s):

1. (Sample Preparation and Characterisation Facility)

Appears in the scientific report 2025

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Database coverage:
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