TY  - JOUR
AU  - Burastero, Osvaldo
AU  - Jones, Nykola C
AU  - Defelipe, Lucas A
AU  - Zavrtanik, Uroš
AU  - Hadži, San
AU  - Hoffmann, Søren Vrønning
AU  - Garcia-Alai, Maria M
TI  - ChiraKit: an online tool for the analysis of circular dichroism spectroscopy data
JO  - Nucleic acids symposium series
VL  - 53
IS  - W1
SN  - 0305-1048
CY  - Oxford
PB  - Oxford Univ. Press
M1  - PUBDB-2025-03100
SP  - W158 - W168
PY  - 2025
N1  - ISSN 1362-4962 not unique: **2 hits**.
AB  - Circular dichroism (CD) spectroscopy is an established biophysical technique to study chiral molecules. CD allows investigating conformational changes under varying experimental conditions and has been used to understand secondary structure, folding, and binding of proteins and nucleic acids. Here, we present ChiraKit, a user-friendly, online, and open-source tool to process raw CD data and perform advanced analysis. ChiraKit features include the calculation of protein secondary structure with the SELCON3 and SESCA algorithms, estimation of peptide helicity using the helix-ensemble model, the fitting of thermal/chemical unfolding or user-defined models, and the decomposition of spectra through singular value decomposition or principal component analysis. ChiraKit can be accessed at https://spc.embl-hamburg.de/. 
LB  - PUB:(DE-HGF)16
C6  - pmid:40287821
DO  - DOI:10.1093/nar/gkaf350
UR  - https://bib-pubdb1.desy.de/record/635345
ER  -