Journal Article

PUBDB-2024-08045

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Pilot screening of potential matrikines resulting from collagen breakages through ionizing radiation

Montanari, J. ; Schwob, L.XFEL.EU*DESY* ; Marie-Brasset, A. ; Vinatier, C. ; Lepleux, C. ; Antoine, R. ; Guicheux, J. ; Poully, J.-C. (Corresponding author)Extern* ; Chevalier, F. (Corresponding author)

2024
Springer New York, NY

This record in other databases:      

Please use a persistent id in citations: doi:10.1007/s00411-024-01086-z  doi:10.3204/PUBDB-2024-08045

Abstract: Little is known regarding radiation-induced matrikines and the possible degradation of extracellular matrix following therapeutic irradiation. The goal of this study was to determine if irradiation can cut collagen proteins at specific sites, inducing potentially biologically active peptides against cartilage cells. Chondrocytes cultured as 3D models were evaluated for extracellular matrix production. Bystander molecules were analyzed in vitro in the conditioned medium of X-irradiated chondrocytes. Preferential breakage sites were analyzed in collagen polypeptide by mass spectrometry and resulting peptides were tested against chondrocytes. 3D models of chondrocytes displayed a light extracellular matrix able to maintain the structure. Irradiated and bystander chondrocytes showed a surprising radiation sensitivity at low doses, characteristic of the presence of bystander factors, particularly following 0.1 Gy. The glycine-proline peptidic bond was observed as a preferential cleavage site and a possible weakness of the collagen polypeptide after irradiation. From the 46 collagen peptides analyzed against chondrocytes culture, 20 peptides induced a reduction of viability and 5 peptides induced an increase of viability at the highest concentration between 0.1 and 1 µg/ml. We conclude that irradiation promoted a site-specific degradation of collagen. The potentially resulting peptides induce negative or positive regulations of chondrocyte growth. Taken together, these results suggest that ionizing radiation causes a degradation of cartilage proteins, leading to a functional unbalance of cartilage homeostasis after exposure, contributing to cartilage dysfunction.

---

Contributing Institute(s):

1. Biomoleküle in Gasphase (FS-BIG)

Research Program(s):

1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)

Experiment(s):

1. Measurement at external facility

Appears in the scientific report 2024

---

Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Springer ; Essential Science Indicators ; IF < 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

---