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@ARTICLE{Montanari:619954,
      author       = {Montanari, Juliette and Schwob, Lucas and Marie-Brasset,
                      Aurélie and Vinatier, Claire and Lepleux, Charlotte and
                      Antoine, Rodolphe and Guicheux, Jérôme and Poully,
                      Jean-Christophe and Chevalier, François},
      title        = {{P}ilot screening of potential matrikines resulting from
                      collagen breakages through ionizing radiation},
      journal      = {Radiation and environmental biophysics},
      volume       = {63},
      number       = {3},
      issn         = {0301-634X},
      address      = {New York, NY},
      publisher    = {Springer},
      reportid     = {PUBDB-2024-08045},
      pages        = {337-350},
      year         = {2024},
      abstract     = {Little is known regarding radiation-induced matrikines and
                      the possible degradation of extracellular matrix following
                      therapeutic irradiation. The goal of this study was to
                      determine if irradiation can cut collagen proteins at
                      specific sites, inducing potentially biologically active
                      peptides against cartilage cells. Chondrocytes cultured as
                      3D models were evaluated for extracellular matrix
                      production. Bystander molecules were analyzed in vitro in
                      the conditioned medium of X-irradiated chondrocytes.
                      Preferential breakage sites were analyzed in collagen
                      polypeptide by mass spectrometry and resulting peptides were
                      tested against chondrocytes. 3D models of chondrocytes
                      displayed a light extracellular matrix able to maintain the
                      structure. Irradiated and bystander chondrocytes showed a
                      surprising radiation sensitivity at low doses,
                      characteristic of the presence of bystander factors,
                      particularly following 0.1 Gy. The glycine-proline peptidic
                      bond was observed as a preferential cleavage site and a
                      possible weakness of the collagen polypeptide after
                      irradiation. From the 46 collagen peptides analyzed against
                      chondrocytes culture, 20 peptides induced a reduction of
                      viability and 5 peptides induced an increase of viability at
                      the highest concentration between 0.1 and 1 µg/ml. We
                      conclude that irradiation promoted a site-specific
                      degradation of collagen. The potentially resulting peptides
                      induce negative or positive regulations of chondrocyte
                      growth. Taken together, these results suggest that ionizing
                      radiation causes a degradation of cartilage proteins,
                      leading to a functional unbalance of cartilage homeostasis
                      after exposure, contributing to cartilage dysfunction.},
      cin          = {FS-BIG},
      ddc          = {530},
      cid          = {I:(DE-H253)FS-BIG-20220318},
      pnm          = {633 - Life Sciences – Building Blocks of Life: Structure
                      and Function (POF4-633)},
      pid          = {G:(DE-HGF)POF4-633},
      experiment   = {EXP:(DE-MLZ)External-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {39115696},
      UT           = {WOS:001286364200001},
      doi          = {10.1007/s00411-024-01086-z},
      url          = {https://bib-pubdb1.desy.de/record/619954},
}