Journal Article

PUBDB-2024-05775

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Drug repurposing screen to identify inhibitors of the RNA polymerase (nsp12) and helicase (nsp13) from SARS-CoV-2 replication and transcription complex

Kuzikov, M. (Corresponding author) ; Reinshagen, J. ; Wycisk, K. ; Corona, A. ; Esposito, F. ; Malune, P. ; Manelfi, C. ; Iaconis, D. ; Beccari, A. ; Tramontano, E. ; Nowotny, M. ; Windshügel, B. ; Gribbon, P. ; Zaliani, A.

2024
Elsevier Amsterdam

This record in other databases:        

Please use a persistent id in citations: doi:10.1016/j.virusres.2024.199356  doi:10.3204/PUBDB-2024-05775

Abstract: Coronaviruses contain one of the largest genomes among the RNA viruses, coding for 14–16 non-structural proteins (nsp) that are involved in proteolytic processing, genome replication and transcription, and four structural proteins that build the core of the mature virion. Due to conservation across coronaviruses, nsps form a group of promising drug targets as their inhibition directly affects viral replication and, therefore, progression of infection. A minimal but fully functional replication and transcription complex was shown to be formed by one RNA-dependent RNA polymerase (nsp12), one nsp7, two nsp8 accessory subunits, and two helicase (nsp13) enzymes. Our approach involved, targeting nsp12 and nsp13 to allow multiple starting point to interfere with virus infection progression. Here we report a combined in-vitro repurposing screening approach, identifying new and confirming reported SARS-CoV-2 nsp12 and nsp13 inhibitors.

Note: ISSN 0168-1702 not unique: **2 hits**.

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Contributing Institute(s):

1. CSSB-CF-SPC (CSSB-CF-SPC)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)
2. BY-COVID - Beyond COVID (101046203) (101046203)
3. EXSCALATE4CoV - EXaSCale smArt pLatform Against paThogEns for Corona Virus (101003551) (101003551)

Experiment(s):

1. No specific instrument

Appears in the scientific report 2024

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Database coverage:
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