Journal Article

PUBDB-2024-04758

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Light-Induced Transformation of Virus-Like Particles on TiO$_2$

Kohantorabi, M. (Corresponding author)DESY* ; Ugolotti ; Sochor, B.DESY* ; Roessler, J. ; Wagstaffe, M.Extern* ; Meinhardt, A.DESY* ; Beck, E. E.DESY* ; Dolling, S.Extern*DESY* ; Blanco Garcia, M.DESY* ; Creutzburg, M.DESY* ; Keller, T. F.XFEL.EU*DESY* ; Schwartzkopf, M.DESY* ; Koyiloth Vayalil, S.Extern*DESY* ; Thünauer, R.Extern*CSSB* ; Guedez, G.EMBL* ; Loew, C.CSSB* ; Ebert, G. ; Protzer ; Hammerschmidt ; Zeidler ; Roth, S.XFEL.EU*DESY* ; Valentin, D. ; Stierle, A.XFEL.EU*DESY* ; Noei, H. (Corresponding author)DESY*

2024
Soc. Washington, DC

This record in other databases:        

Please use a persistent id in citations: doi:10.1021/acsami.4c07151  doi:10.3204/PUBDB-2024-04758

Abstract: Titanium dioxide (TiO$_2$) shows significant potential as a self-cleaning material to inactivate the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and prevent virus transmission. This study provides insights into the impact of UV-A light on the photocatalytic inactivation of adsorbed SARS-CoV-2 virus-like particles (VLPs) on a TiO$_2$ surface at the molecular and atomic levels. X-ray photoelectron spectroscopy, combined with density functional theory calculations, reveals that spike proteins can adsorb on TiO$_2$ predominantly via their amine and amide functional groups in their amino acids blocks. We employ atomic force microscopy and grazing-incidence small-angle X-ray scattering (GISAXS) to investigate the molecular-scale morphological changes during the inactivation of VLPs on TiO$_2$ under light irradiation. Notably, in-situ measurements reveal photo-induced morphological changes of VLPs, resulting in an increased particle diameters. These results suggest that denaturation of structural proteins induced by UV irradiation and oxidation of the virus structure through photocatalytic reactions can take place on TiO$_2$ surface. The in-situ GISAXS measurements under N$_2$ atmosphere reveal that the virus morphology remains intact under UV light. This provides evidence that the presence of both oxygen and UV light is necessary to initiate photocatalytic reactions on the surface and subsequently inactivate the adsorbed viruses. The chemical insights into the virus inactivation process obtained in this study contribute significantly to the development of solid materials for inactivation of enveloped viruses.

Note: L:MB

---

Contributing Institute(s):

1. Nanolab (FS-NL)
2. CSSB-EMBL-CL (CSSB-EMBL-CL)
3. CSSB-CF-ALFM (CSSB-CF-ALFM)

Research Program(s):

1. 632 - Materials – Quantum, Complex and Functional Materials (POF4-632) (POF4-632)
2. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
3. FS-Proposal: I-20211020 (I-20211020) (I-20211020)
4. FS-Proposal: I-20230640 (I-20230640) (I-20230640)

Experiment(s):

1. DESY NanoLab: Microscopy
2. DESY NanoLab: Sample Preparation
3. DESY NanoLab: Surface Spectroscopy
4. PETRA Beamline P03 (PETRA III)
5. (Advanced Light and Fluorescence Microscopy Facility)

Appears in the scientific report 2024

---

Database coverage:
; ; ; Clarivate Analytics Master Journal List ; Current Contents - Engineering, Computing and Technology ; Current Contents - Physical, Chemical and Earth Sciences ; Essential Science Indicators ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

---