Journal Article

PUBDB-2024-00514

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png The Cryo-EM structures of two amphibian antimicrobial cross-β amyloid fibrils

Bücker, R.CFEL*CSSB* ; Seuring, C.CFEL*CSSB*XFEL.EU* ; Cazey, C.CSSB* ; Veith, K.Extern* ; García-Alai, M. ; Gruenewald, K. (Corresponding author)CSSB*DESY* ; Landau, M. (Corresponding author)CSSB*EMBL*DESY*

2022
Nature Publishing Group UK [London]

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Please use a persistent id in citations: doi:10.1038/s41467-022-32039-z  doi:10.3204/PUBDB-2024-00514

Abstract: The amyloid-antimicrobial link hypothesis is based on antimicrobial properties found in human amyloids involved in neurodegenerative and systemic diseases, along with amyloidal structural properties found in antimicrobial peptides (AMPs). Supporting this hypothesis, we here determined the fibril structure of two AMPs from amphibians, uperin 3.5 and aurein 3.3, by cryogenic electron microscopy (cryo-EM), revealing amyloid cross-β fibrils of mated β-sheets at atomic resolution. Uperin 3.5 formed a 3-blade symmetrical propeller of nine peptides per fibril layer including tight β-sheet interfaces. This cross-β cryo-EM structure complements the cross-α fibril conformation previously determined by crystallography, substantiating a secondary structure switch mechanism of uperin 3.5. The aurein 3.3 arrangement consisted of six peptides per fibril layer, all showing kinked β-sheets allowing a rounded compactness of the fibril. The kinked β-sheets are similar to LARKS (Low-complexity, Amyloid-like, Reversible, Kinked Segments) found in human functional amyloids.

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Contributing Institute(s):

1. CSSB - Leibniz-Institut für Experimentelle Virologie (LIV) - Kay Grünewald (CSSB-LIV-KG)
2. CSSB-CF-CRYO (CSSB-CF-CRYO)
3. FPF Functional Protein Fibrils (CSSB-F)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Experiment(s):

1. No specific instrument

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