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@ARTICLE{Bcker:602188,
author = {Bücker, Robert and Seuring, Carolin and Cazey, Cornelia
and Veith, Katharina and García-Alai, Maria and Gruenewald,
Kay and Landau, Meytal},
title = {{T}he {C}ryo-{EM} structures of two amphibian antimicrobial
cross-β amyloid fibrils},
journal = {Nature Communications},
volume = {13},
number = {1},
issn = {2041-1723},
address = {[London]},
publisher = {Nature Publishing Group UK},
reportid = {PUBDB-2024-00514},
pages = {4356},
year = {2022},
abstract = {The amyloid-antimicrobial link hypothesis is based on
antimicrobial properties found in human amyloids involved in
neurodegenerative and systemic diseases, along with
amyloidal structural properties found in antimicrobial
peptides (AMPs). Supporting this hypothesis, we here
determined the fibril structure of two AMPs from amphibians,
uperin 3.5 and aurein 3.3, by cryogenic electron microscopy
(cryo-EM), revealing amyloid cross-β fibrils of mated
β-sheets at atomic resolution. Uperin 3.5 formed a 3-blade
symmetrical propeller of nine peptides per fibril layer
including tight β-sheet interfaces. This cross-β cryo-EM
structure complements the cross-α fibril conformation
previously determined by crystallography, substantiating a
secondary structure switch mechanism of uperin 3.5. The
aurein 3.3 arrangement consisted of six peptides per fibril
layer, all showing kinked β-sheets allowing a rounded
compactness of the fibril. The kinked β-sheets are similar
to LARKS (Low-complexity, Amyloid-like, Reversible, Kinked
Segments) found in human functional amyloids.},
cin = {CSSB-LIV-KG / CSSB-CF-CRYO / CSSB-F},
ddc = {500},
cid = {I:(DE-H253)CSSB-LIV-KG-20220525 /
I:(DE-H253)CSSB-CF-CRYO-20210520 /
I:(DE-H253)CSSB-F-20230420},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
experiment = {EXP:(DE-MLZ)NOSPEC-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:35896552},
UT = {WOS:000831732000020},
doi = {10.1038/s41467-022-32039-z},
url = {https://bib-pubdb1.desy.de/record/602188},
}