Journal Article

PUBDB-2024-00238

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Disruption of a Plasmodium falciparum patatin‐like phospholipase delays male gametocyte exflagellation

Pietsch, E.CSSB* ; Niedermüller, K. ; Andrews, M. ; Meyer, B. S. ; Lenz, T. L. ; Wilson, D.CSSB* ; Gilberger, T. (Corresponding author)CSSB* ; Burda, P.-C. (Corresponding author)CSSB*

2024
Wiley-Blackwell Oxford [u.a.]

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Please use a persistent id in citations: doi:10.1111/mmi.15211  doi:10.3204/PUBDB-2024-00238

Abstract: An essential process in transmission of the malaria parasite to the Anopheles vector is the conversion of mature gametocytes into gametes within the mosquito gut, where they egress from the red blood cell (RBC). During egress, male gametocytes undergo exflagellation, leading to the formation of eight haploid motile microgametes, while female gametes retain their spherical shape. Gametocyte egress depends on sequential disruption of the parasitophorous vacuole membrane and the host cell membrane. In other life cycle stages of the malaria parasite, phospholipases have been implicated in membrane disruption processes during egress, however their importance for gametocyte egress is relatively unknown. Here, we performed comprehensive functional analyses of six putative phospholipases for their role during development and egress of Plasmodium falciparum gametocytes. We localize two of them, the prodrug activation and resistance esterase (PF3D7_0709700) and the lysophospholipase 1 (PF3D7_1476700), to the parasite plasma membrane. Subsequently, we show that disruption of most of the studied phospholipase genes does neither affect gametocyte development nor egress. The exception is the putative patatin-like phospholipase 3 (PF3D7_0924000), whose gene deletion leads to a delay in male gametocyte exflagellation, indicating an important, albeit not essential, role of this enzyme in male gametogenesis.

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Contributing Institute(s):

1. CSSB-BNITM-TG (CSSB-BNITM-TG)
2. CSSB-CF-ALFM (CSSB-CF-ALFM)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)
2. DFG project G:(GEPRIS)414222880 - Funktionelle Charakterisierung der Phospholipasen im Malariaerreger Plasmodium falciparum (414222880) (414222880)
3. DFG project 446556156 - Identifizierung und Charakterisierung neuer Faktoren, welche die Freisetzung von Plasmodium falciparum aus roten Blutzellen ermöglichen (446556156) (446556156)

Experiment(s):

1. No specific instrument

Appears in the scientific report 2024

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Wiley ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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