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@ARTICLE{Pietsch:601529,
      author       = {Pietsch, Emma and Niedermüller, Korbinian and Andrews, Mia
                      and Meyer, Britta S. and Lenz, Tobias L. and Wilson, Danny
                      and Gilberger, Tim and Burda, Paul-Christian},
      title        = {{D}isruption of a {P}lasmodium falciparum patatin‐like
                      phospholipase delays male gametocyte exflagellation},
      journal      = {Molecular microbiology},
      volume       = {121},
      number       = {3},
      issn         = {0950-382X},
      address      = {Oxford [u.a.]},
      publisher    = {Wiley-Blackwell},
      reportid     = {PUBDB-2024-00238},
      pages        = {529-542},
      year         = {2024},
      abstract     = {An essential process in transmission of the malaria
                      parasite to the Anopheles vector is the conversion of mature
                      gametocytes into gametes within the mosquito gut, where they
                      egress from the red blood cell (RBC). During egress, male
                      gametocytes undergo exflagellation, leading to the formation
                      of eight haploid motile microgametes, while female gametes
                      retain their spherical shape. Gametocyte egress depends on
                      sequential disruption of the parasitophorous vacuole
                      membrane and the host cell membrane. In other life cycle
                      stages of the malaria parasite, phospholipases have been
                      implicated in membrane disruption processes during egress,
                      however their importance for gametocyte egress is relatively
                      unknown. Here, we performed comprehensive functional
                      analyses of six putative phospholipases for their role
                      during development and egress of Plasmodium falciparum
                      gametocytes. We localize two of them, the prodrug activation
                      and resistance esterase $(PF3D7_0709700)$ and the
                      lysophospholipase 1 $(PF3D7_1476700),$ to the parasite
                      plasma membrane. Subsequently, we show that disruption of
                      most of the studied phospholipase genes does neither affect
                      gametocyte development nor egress. The exception is the
                      putative patatin-like phospholipase 3 $(PF3D7_0924000),$
                      whose gene deletion leads to a delay in male gametocyte
                      exflagellation, indicating an important, albeit not
                      essential, role of this enzyme in male gametogenesis.},
      cin          = {CSSB-BNITM-TG / CSSB-CF-ALFM},
      ddc          = {570},
      cid          = {I:(DE-H253)CSSB-BNITM-TG-20210520 /
                      I:(DE-H253)CSSB-CF-ALFM-20210629},
      pnm          = {899 - ohne Topic (POF4-899) / DFG project
                      G:(GEPRIS)414222880 - Funktionelle Charakterisierung der
                      Phospholipasen im Malariaerreger Plasmodium falciparum
                      (414222880) / DFG project 446556156 - Identifizierung und
                      Charakterisierung neuer Faktoren, welche die Freisetzung von
                      Plasmodium falciparum aus roten Blutzellen ermöglichen
                      (446556156)},
      pid          = {G:(DE-HGF)POF4-899 / G:(GEPRIS)414222880 /
                      G:(GEPRIS)446556156},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:38131156},
      UT           = {WOS:001128899200001},
      doi          = {10.1111/mmi.15211},
      url          = {https://bib-pubdb1.desy.de/record/601529},
}