Journal Article

PUBDB-2024-00102

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Guided-deconvolution for correlative light and electron microscopy

Ma, F. ; Kaufmann, R.CSSB* ; Sedzicki, J. ; Cseresnyés, Z. ; Dehio, C. ; Hoeppener, S. ; Figge, M. T. ; Heintzmann, R. (Corresponding author)

2023
PLOS San Francisco, California, US

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Please use a persistent id in citations: doi:10.1371/journal.pone.0282803  doi:10.3204/PUBDB-2024-00102

Abstract: Correlative light and electron microscopy is a powerful tool to study the internal structure of cells. It combines the mutual benefit of correlating light (LM) and electron (EM) microscopy information. The EM images only contain contrast information. Therefore, some of the detailed structures cannot be specified from these images alone, especially when different cell organelle are contacted. However, the classical approach of overlaying LM onto EM images to assign functional to structural information is hampered by the large discrepancy in structural detail visible in the LM images. This paper aims at investigating an optimized approach which we call EM-guided deconvolution. This applies to living cells structures before fixation as well as previously fixed sample. It attempts to automatically assign fluorescence-labeled structures to structural details visible in the EM image to bridge the gaps in both resolution and specificity between the two imaging modes. We tested our approach on simulations, correlative data of multi-color beads and previously published data of biological samples.

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Contributing Institute(s):

1. CSSB-UHH-RK (CSSB-UHH-RK)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)
2. DFG project G:(GEPRIS)390713860 - EXC 2051: Balance of the Microverse (390713860) (390713860)
3. SFB 1278 C04 - Untersuchung zellulärer Antworten auf Nanopartikelaufnahme mithilfe von korrelativer TEM und hochaufgelöster Fluoreszenzabbildung (C04) (388042957) (388042957)

Experiment(s):

1. No specific instrument

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