Journal Article PUBDB-2023-06274

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Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS)

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2023
Springer Nature London

Communications biology 6(1), 1057 () [10.1038/s42003-023-05416-7]
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Abstract: Free-electron lasers (FEL) are revolutionizing X-ray-based structural biology methods. While protein crystallography is already routinely performed at FELs, Small Angle X-ray Scattering (SAXS) studies of biological macromolecules are not as prevalent. SAXS allows the study of the shape and overall structure of proteins and nucleic acids in solution, in a quasi-native environment. In solution, chemical and biophysical parameters that have an influence on the structure and dynamics of molecules can be varied and their effect on conformational changes can be monitored in time-resolved XFEL and SAXS experiments. We report here the collection of scattering form factors of proteins in solution using FEL X-rays. The form factors correspond to the scattering signal of the protein ensemble alone; the scattering contributions from the solvent and the instrument are separately measured and accurately subtracted. The experiment was done using a liquid jet for sample delivery. These results pave the way for time-resolved studies and measurements from dilute samples, capitalizing on the intense and short FEL X-ray pulses.

Classification:

Contributing Institute(s):
  1. CFEL-Coherent X-Ray Imaging (FS-CFEL-1)
  2. SPB/SFX (XFEL_E1_SPB/SFX)
  3. EMBL (EMBL)
  4. Computational Nanoscale Imaging (CFEL-CNI)
  5. Sample Environment and Characterisation (XFEL_E2_SEC)
  6. FS-Arbeitsgruppe (FS-ML)
  7. Strukturelle Mikrobiologie CSSB (FS-CS)
  8. CSSB-EMBL-CL (CSSB-EMBL-CL)
Research Program(s):
  1. 633 - Life Sciences – Building Blocks of Life: Structure and Function (POF4-633) (POF4-633)
  2. 6G3 - PETRA III (DESY) (POF4-6G3) (POF4-6G3)
  3. DFG project 390715994 - EXC 2056: CUI: Advanced Imaging of Matter (390715994) (390715994)
Experiment(s):
  1. Experiments at XFEL
  2. PETRA Beamline P12 (PETRA III)

Appears in the scientific report 2023
Database coverage:
Medline ; Creative Commons Attribution CC BY 4.0 ; DOAJ ; OpenAccess ; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DOAJ Seal ; Ebsco Academic Search ; IF >= 5 ; JCR ; PubMed Central ; SCOPUS ; Web of Science Core Collection ; Zoological Record
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The record appears in these collections:
Private Collections > >CFEL > >FS-CFEL > CFEL-CNI
Private Collections > >DESY > >FS > FS-CFEL-1
Private Collections > >DESY > >FS > FS-CS
Private Collections > >DESY > >FS > FS-ML
Private Collections > >XFEL.EU > XFEL_E1_SPB/SFX
Private Collections > >CSSB > CSSB-EMBL-CL
Private Collections > >XFEL.EU > XFEL_E2_SEC
Document types > Articles > Journal Article
Private Collections > >EMBL > EMBL
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 Record created 2023-10-19, last modified 2025-07-15


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