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@ARTICLE{Blanchet:596784,
author = {Blanchet, Clement and Round, Adam and Mertens, Haydyn D. T.
and Ayyer, Kartik and Gräwert, Melissa Ann and Awel, Salah
and Franke, Daniel and Dörner, Katerina and Bajt, Saša and
Bean, Richard and Custódio, Tânia F. and de Wijn, Raphael
and Juncheng, E. and Henkel, Alessandra and Gruzinov, Andrey
and Jeffries, Cy M. and Kim, Yoonhee and Kirkwood, Henry and
Kloos, Marco and Knoška, Juraj and Koliyadu, Jayanath and
Letrun, Romain and Loew, Christian and Makroczyova, Jana and
Mall, Abhishek and Meijers, Rob and Pena Murillo, Gisel
Esperanza and Oberthür, Dominik and Round, Ekaterina and
Seuring, Carolin and Sikorski, Marcin and Vagovic, Patrik
and Valerio, Joana and Wollweber, Tamme and Zhuang, Yulong
and Schulz, Joachim and Haas, Heinrich and Chapman, Henry N.
and Mancuso, Adrian P. and Svergun, Dmitri},
title = {{F}orm factor determination of biological molecules with
{X}-ray free electron laser small-angle scattering
({XFEL}-{SAS})},
journal = {Communications biology},
volume = {6},
number = {1},
issn = {2399-3642},
address = {London},
publisher = {Springer Nature},
reportid = {PUBDB-2023-06274},
pages = {1057},
year = {2023},
abstract = {Free-electron lasers (FEL) are revolutionizing X-ray-based
structural biology methods. While protein crystallography is
already routinely performed at FELs, Small Angle X-ray
Scattering (SAXS) studies of biological macromolecules are
not as prevalent. SAXS allows the study of the shape and
overall structure of proteins and nucleic acids in solution,
in a quasi-native environment. In solution, chemical and
biophysical parameters that have an influence on the
structure and dynamics of molecules can be varied and their
effect on conformational changes can be monitored in
time-resolved XFEL and SAXS experiments. We report here the
collection of scattering form factors of proteins in
solution using FEL X-rays. The form factors correspond to
the scattering signal of the protein ensemble alone; the
scattering contributions from the solvent and the instrument
are separately measured and accurately subtracted. The
experiment was done using a liquid jet for sample delivery.
These results pave the way for time-resolved studies and
measurements from dilute samples, capitalizing on the
intense and short FEL X-ray pulses.},
cin = {FS-CFEL-1 / $XFEL_E1_SPB/SFX$ / EMBL / CFEL-CNI /
$XFEL_E2_SEC$ / FS-ML / FS-CS / CSSB-EMBL-CL},
ddc = {570},
cid = {I:(DE-H253)FS-CFEL-1-20120731 /
$I:(DE-H253)XFEL_E1_SPB_SFX-20210408$ /
I:(DE-H253)EMBL-20120731 / I:(DE-H253)CFEL-CNI-20190417 /
$I:(DE-H253)XFEL_E2_SEC-20210408$ /
I:(DE-H253)FS-ML-20120731 / I:(DE-H253)FS-CS-20210408 /
I:(DE-H253)CSSB-EMBL-CL-20210806},
pnm = {633 - Life Sciences – Building Blocks of Life: Structure
and Function (POF4-633) / 6G3 - PETRA III (DESY) (POF4-6G3)
/ DFG project 390715994 - EXC 2056: CUI: Advanced Imaging of
Matter (390715994)},
pid = {G:(DE-HGF)POF4-633 / G:(DE-HGF)POF4-6G3 /
G:(GEPRIS)390715994},
experiment = {EXP:(DE-H253)XFEL-Exp-20150101 /
EXP:(DE-H253)P-P12-20150101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:001115957900003},
doi = {10.1038/s42003-023-05416-7},
url = {https://bib-pubdb1.desy.de/record/596784},
}